Electrostatic Ligand Coatings of Nanoparticles Enable Ligand-Specific Gene Delivery to Human Primary Cells

A general method of coating polymer/DNA nanoparticles was developed. Peptide coated nanoparticles were found to have favorable biophysical characteristics including small particle size, near-neutral ζ potential, and stability in serum. At appropriate formulation conditions including near-neutral cha...

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Bibliographic Details
Published in:Nano letters 2007-04, Vol.7 (4), p.874-879
Main Authors: Green, Jordan J, Chiu, Eugene, Leshchiner, Elizaveta S, Shi, Julie, Langer, Robert, Anderson, Daniel G
Format: Article
Language:English
Subjects:
Cells, Cultured
Coated Materials, Biocompatible - chemistry
Cross-disciplinary physics: materials science
rheology
DNA - administration & dosage
DNA - chemistry
DNA - pharmacokinetics
Drug Delivery Systems - methods
Electroporation - methods
Endothelial Cells - metabolism
Exact sciences and technology
Gene Targeting - methods
Gene Transfer Techniques
Humans
Ligands
Materials science
Nanocrystalline materials
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Nanoscale materials and structures: fabrication and characterization
Particle Size
Peptides - chemistry
Physics
Static Electricity
Transfection - methods
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Summary:A general method of coating polymer/DNA nanoparticles was developed. Peptide coated nanoparticles were found to have favorable biophysical characteristics including small particle size, near-neutral ζ potential, and stability in serum. At appropriate formulation conditions including near-neutral charge ratio, the coated nanoparticles enabled effective ligand-specific gene delivery to human primary endothelial cells in serum-containing media. As this nanoparticulate drug delivery system has high efficacy, ligand-based specificity, biodegradability, and low cytotoxicity, it may be potentially useful in several clinical applications.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl062395b