Loading…

Electrostatic Ligand Coatings of Nanoparticles Enable Ligand-Specific Gene Delivery to Human Primary Cells

A general method of coating polymer/DNA nanoparticles was developed. Peptide coated nanoparticles were found to have favorable biophysical characteristics including small particle size, near-neutral ζ potential, and stability in serum. At appropriate formulation conditions including near-neutral cha...

Full description

Saved in:

Bibliographic Details
Published in:	Nano letters 2007-04, Vol.7 (4), p.874-879
Main Authors:	Green, Jordan J, Chiu, Eugene, Leshchiner, Elizaveta S, Shi, Julie, Langer, Robert, Anderson, Daniel G
Format:	Article
Language:	English
Subjects:	Cells, Cultured Coated Materials, Biocompatible - chemistry Cross-disciplinary physics: materials science rheology DNA - administration & dosage DNA - chemistry DNA - pharmacokinetics Drug Delivery Systems - methods Electroporation - methods Endothelial Cells - metabolism Exact sciences and technology Gene Targeting - methods Gene Transfer Techniques Humans Ligands Materials science Nanocrystalline materials Nanoparticles - chemistry Nanoparticles - ultrastructure Nanoscale materials and structures: fabrication and characterization Particle Size Peptides - chemistry Physics Static Electricity Transfection - methods
Citations:	Items that this one cites Items that cite this one
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

cited_by	cdi_FETCH-LOGICAL-a343t-8be5d13077ca6511013879964ae50f3492b271820bdf03b56ffd3ad24d99ba843
cites	cdi_FETCH-LOGICAL-a343t-8be5d13077ca6511013879964ae50f3492b271820bdf03b56ffd3ad24d99ba843
container_end_page	879
container_issue	4
container_start_page	874
container_title	Nano letters
container_volume	7
creator	Green, Jordan J Chiu, Eugene Leshchiner, Elizaveta S Shi, Julie Langer, Robert Anderson, Daniel G
description	A general method of coating polymer/DNA nanoparticles was developed. Peptide coated nanoparticles were found to have favorable biophysical characteristics including small particle size, near-neutral ζ potential, and stability in serum. At appropriate formulation conditions including near-neutral charge ratio, the coated nanoparticles enabled effective ligand-specific gene delivery to human primary endothelial cells in serum-containing media. As this nanoparticulate drug delivery system has high efficacy, ligand-based specificity, biodegradability, and low cytotoxicity, it may be potentially useful in several clinical applications.
doi_str_mv	10.1021/nl062395b
format	article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70369480</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70369480</sourcerecordid><originalsourceid>FETCH-LOGICAL-a343t-8be5d13077ca6511013879964ae50f3492b271820bdf03b56ffd3ad24d99ba843</originalsourceid><addsrcrecordid>eNpt0M9v2yAUB3BUrWrSrIf-AxWXTerB7cNgDMcpy5pJUVtp69l6xhA5IjgzdqX-92OKlVx24teHx-NLyC2DBwY5ewweZM51UV-QOSs4ZFLr_NNprsSMXMe4AwDNC7giM1ZymYOQc7JbeWuGvosDDq2hm3aLoaHLLq3CNtLO0WcM3QH7dOptpKuAtbeTy34drGlduvdkg6XfrW_fbf9Bh46uxz0G-tq3e0wbS-t9_EwuHfpob6ZxQd5-rH4v19nm5enn8tsmQy74kKnaFg3jUJYGZcEYMK5KraVAW4DjQud1XjKVQ9044HUhnWs4NrlotK5RCb4gX491D333Z7RxqPZtNKkDDLYbY1UCl1ooSPD-CE36f-ytqw7HfisG1b9gq1Owyd5NRcd6b5uznJJM4MsEMBr0rsdg2nh2SiophTo7NLHadWMfUhb_efAveL-Lvg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70369480</pqid></control><display><type>article</type><title>Electrostatic Ligand Coatings of Nanoparticles Enable Ligand-Specific Gene Delivery to Human Primary Cells</title><source>American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)</source><creator>Green, Jordan J ; Chiu, Eugene ; Leshchiner, Elizaveta S ; Shi, Julie ; Langer, Robert ; Anderson, Daniel G</creator><creatorcontrib>Green, Jordan J ; Chiu, Eugene ; Leshchiner, Elizaveta S ; Shi, Julie ; Langer, Robert ; Anderson, Daniel G</creatorcontrib><description>A general method of coating polymer/DNA nanoparticles was developed. Peptide coated nanoparticles were found to have favorable biophysical characteristics including small particle size, near-neutral ζ potential, and stability in serum. At appropriate formulation conditions including near-neutral charge ratio, the coated nanoparticles enabled effective ligand-specific gene delivery to human primary endothelial cells in serum-containing media. As this nanoparticulate drug delivery system has high efficacy, ligand-based specificity, biodegradability, and low cytotoxicity, it may be potentially useful in several clinical applications.</description><identifier>ISSN: 1530-6984</identifier><identifier>EISSN: 1530-6992</identifier><identifier>DOI: 10.1021/nl062395b</identifier><identifier>PMID: 17362046</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Cells, Cultured ; Coated Materials, Biocompatible - chemistry ; Cross-disciplinary physics: materials science; rheology ; DNA - administration & dosage ; DNA - chemistry ; DNA - pharmacokinetics ; Drug Delivery Systems - methods ; Electroporation - methods ; Endothelial Cells - metabolism ; Exact sciences and technology ; Gene Targeting - methods ; Gene Transfer Techniques ; Humans ; Ligands ; Materials science ; Nanocrystalline materials ; Nanoparticles - chemistry ; Nanoparticles - ultrastructure ; Nanoscale materials and structures: fabrication and characterization ; Particle Size ; Peptides - chemistry ; Physics ; Static Electricity ; Transfection - methods</subject><ispartof>Nano letters, 2007-04, Vol.7 (4), p.874-879</ispartof><rights>Copyright © 2007 American Chemical Society</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a343t-8be5d13077ca6511013879964ae50f3492b271820bdf03b56ffd3ad24d99ba843</citedby><cites>FETCH-LOGICAL-a343t-8be5d13077ca6511013879964ae50f3492b271820bdf03b56ffd3ad24d99ba843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18686648$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17362046$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Green, Jordan J</creatorcontrib><creatorcontrib>Chiu, Eugene</creatorcontrib><creatorcontrib>Leshchiner, Elizaveta S</creatorcontrib><creatorcontrib>Shi, Julie</creatorcontrib><creatorcontrib>Langer, Robert</creatorcontrib><creatorcontrib>Anderson, Daniel G</creatorcontrib><title>Electrostatic Ligand Coatings of Nanoparticles Enable Ligand-Specific Gene Delivery to Human Primary Cells</title><title>Nano letters</title><addtitle>Nano Lett</addtitle><description>A general method of coating polymer/DNA nanoparticles was developed. Peptide coated nanoparticles were found to have favorable biophysical characteristics including small particle size, near-neutral ζ potential, and stability in serum. At appropriate formulation conditions including near-neutral charge ratio, the coated nanoparticles enabled effective ligand-specific gene delivery to human primary endothelial cells in serum-containing media. As this nanoparticulate drug delivery system has high efficacy, ligand-based specificity, biodegradability, and low cytotoxicity, it may be potentially useful in several clinical applications.</description><subject>Cells, Cultured</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Cross-disciplinary physics: materials science; rheology</subject><subject>DNA - administration & dosage</subject><subject>DNA - chemistry</subject><subject>DNA - pharmacokinetics</subject><subject>Drug Delivery Systems - methods</subject><subject>Electroporation - methods</subject><subject>Endothelial Cells - metabolism</subject><subject>Exact sciences and technology</subject><subject>Gene Targeting - methods</subject><subject>Gene Transfer Techniques</subject><subject>Humans</subject><subject>Ligands</subject><subject>Materials science</subject><subject>Nanocrystalline materials</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - ultrastructure</subject><subject>Nanoscale materials and structures: fabrication and characterization</subject><subject>Particle Size</subject><subject>Peptides - chemistry</subject><subject>Physics</subject><subject>Static Electricity</subject><subject>Transfection - methods</subject><issn>1530-6984</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNpt0M9v2yAUB3BUrWrSrIf-AxWXTerB7cNgDMcpy5pJUVtp69l6xhA5IjgzdqX-92OKlVx24teHx-NLyC2DBwY5ewweZM51UV-QOSs4ZFLr_NNprsSMXMe4AwDNC7giM1ZymYOQc7JbeWuGvosDDq2hm3aLoaHLLq3CNtLO0WcM3QH7dOptpKuAtbeTy34drGlduvdkg6XfrW_fbf9Bh46uxz0G-tq3e0wbS-t9_EwuHfpob6ZxQd5-rH4v19nm5enn8tsmQy74kKnaFg3jUJYGZcEYMK5KraVAW4DjQud1XjKVQ9044HUhnWs4NrlotK5RCb4gX491D333Z7RxqPZtNKkDDLYbY1UCl1ooSPD-CE36f-ytqw7HfisG1b9gq1Owyd5NRcd6b5uznJJM4MsEMBr0rsdg2nh2SiophTo7NLHadWMfUhb_efAveL-Lvg</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Green, Jordan J</creator><creator>Chiu, Eugene</creator><creator>Leshchiner, Elizaveta S</creator><creator>Shi, Julie</creator><creator>Langer, Robert</creator><creator>Anderson, Daniel G</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070401</creationdate><title>Electrostatic Ligand Coatings of Nanoparticles Enable Ligand-Specific Gene Delivery to Human Primary Cells</title><author>Green, Jordan J ; Chiu, Eugene ; Leshchiner, Elizaveta S ; Shi, Julie ; Langer, Robert ; Anderson, Daniel G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a343t-8be5d13077ca6511013879964ae50f3492b271820bdf03b56ffd3ad24d99ba843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Cells, Cultured</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>Cross-disciplinary physics: materials science; rheology</topic><topic>DNA - administration & dosage</topic><topic>DNA - chemistry</topic><topic>DNA - pharmacokinetics</topic><topic>Drug Delivery Systems - methods</topic><topic>Electroporation - methods</topic><topic>Endothelial Cells - metabolism</topic><topic>Exact sciences and technology</topic><topic>Gene Targeting - methods</topic><topic>Gene Transfer Techniques</topic><topic>Humans</topic><topic>Ligands</topic><topic>Materials science</topic><topic>Nanocrystalline materials</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - ultrastructure</topic><topic>Nanoscale materials and structures: fabrication and characterization</topic><topic>Particle Size</topic><topic>Peptides - chemistry</topic><topic>Physics</topic><topic>Static Electricity</topic><topic>Transfection - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Green, Jordan J</creatorcontrib><creatorcontrib>Chiu, Eugene</creatorcontrib><creatorcontrib>Leshchiner, Elizaveta S</creatorcontrib><creatorcontrib>Shi, Julie</creatorcontrib><creatorcontrib>Langer, Robert</creatorcontrib><creatorcontrib>Anderson, Daniel G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nano letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Green, Jordan J</au><au>Chiu, Eugene</au><au>Leshchiner, Elizaveta S</au><au>Shi, Julie</au><au>Langer, Robert</au><au>Anderson, Daniel G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrostatic Ligand Coatings of Nanoparticles Enable Ligand-Specific Gene Delivery to Human Primary Cells</atitle><jtitle>Nano letters</jtitle><addtitle>Nano Lett</addtitle><date>2007-04-01</date><risdate>2007</risdate><volume>7</volume><issue>4</issue><spage>874</spage><epage>879</epage><pages>874-879</pages><issn>1530-6984</issn><eissn>1530-6992</eissn><abstract>A general method of coating polymer/DNA nanoparticles was developed. Peptide coated nanoparticles were found to have favorable biophysical characteristics including small particle size, near-neutral ζ potential, and stability in serum. At appropriate formulation conditions including near-neutral charge ratio, the coated nanoparticles enabled effective ligand-specific gene delivery to human primary endothelial cells in serum-containing media. As this nanoparticulate drug delivery system has high efficacy, ligand-based specificity, biodegradability, and low cytotoxicity, it may be potentially useful in several clinical applications.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>17362046</pmid><doi>10.1021/nl062395b</doi><tpages>6</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 1530-6984
ispartof	Nano letters, 2007-04, Vol.7 (4), p.874-879
issn	1530-6984 1530-6992
language	eng
recordid	cdi_proquest_miscellaneous_70369480
source	American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects	Cells, Cultured Coated Materials, Biocompatible - chemistry Cross-disciplinary physics: materials science rheology DNA - administration & dosage DNA - chemistry DNA - pharmacokinetics Drug Delivery Systems - methods Electroporation - methods Endothelial Cells - metabolism Exact sciences and technology Gene Targeting - methods Gene Transfer Techniques Humans Ligands Materials science Nanocrystalline materials Nanoparticles - chemistry Nanoparticles - ultrastructure Nanoscale materials and structures: fabrication and characterization Particle Size Peptides - chemistry Physics Static Electricity Transfection - methods
title	Electrostatic Ligand Coatings of Nanoparticles Enable Ligand-Specific Gene Delivery to Human Primary Cells
url	http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T10%3A14%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Electrostatic%20Ligand%20Coatings%20of%20Nanoparticles%20Enable%20Ligand-Specific%20Gene%20Delivery%20to%20Human%20Primary%20Cells&rft.jtitle=Nano%20letters&rft.au=Green,%20Jordan%20J&rft.date=2007-04-01&rft.volume=7&rft.issue=4&rft.spage=874&rft.epage=879&rft.pages=874-879&rft.issn=1530-6984&rft.eissn=1530-6992&rft_id=info:doi/10.1021/nl062395b&rft_dat=%3Cproquest_cross%3E70369480%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-a343t-8be5d13077ca6511013879964ae50f3492b271820bdf03b56ffd3ad24d99ba843%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=70369480&rft_id=info:pmid/17362046&rfr_iscdi=true