Separation, characterization and identification of boar seminal plasma proteins

Methods used for the isolation, separation and characterization of boar seminal plasma proteins are discussed, as well as techniques applied to study their binding properties. Attention is paid to interactions of these proteins with different types of saccharides and glycoconjugates, with membrane p...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2007-04, Vol.849 (1), p.307-314
Main Authors: JONAKOVA, V, MAFIISKOVA, P, TICHA, M
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Animals
Binding properties of proteins
Biological and medical sciences
Boar seminal plasma proteins
Chromatography, Affinity
Chromatography, Gel
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
General pharmacology
Identification and characterization of proteins
Male
Medical sciences
Pharmacology. Drug treatments
Protein Binding
Reproducibility of Results
Reproduction
Seminal Plasma Proteins - analysis
Seminal Plasma Proteins - isolation & purification
Seminal Plasma Proteins - metabolism
Separation methods
Sperm Capacitation
Spermadhesins
Swine
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Summary:Methods used for the isolation, separation and characterization of boar seminal plasma proteins are discussed, as well as techniques applied to study their binding properties. Attention is paid to interactions of these proteins with different types of saccharides and glycoconjugates, with membrane phospholipids, and to interactions between proteins. Boar seminal plasma contains different types of proteins: spermadhesins of the AQN and AWN families; DQH and PSP proteins belong to the most abundant. Some of these proteins are bound to the sperm surface during ejaculation and thus protein-coating layers of sperm are formed. Sperms coated with proteins participate in different types of interactions occurring in the course of the reproduction process, e.g. formation of the oviductal sperm reservoir, sperm capacitation, oocyte recognition and sperm binding to the oocyte.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2006.10.054