Comparative proteomics of rat liver and Morris hepatoma 7777 plasma membranes

Plasma membranes from normal rat liver and hepatocellular carcinoma Morris hepatoma 7777 were selectively solubilized by use of different reagents. After selective solubilization, proteins were identified by nano-HPLC–electrospray ionization tandem mass spectrometry (LC-ESI MS/MS). Using simple soft...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2007-04, Vol.849 (1), p.293-301
Main Authors: Clifton, James G., Li, Xuesong, Reutter, Werner, Hixson, Douglas C., Josic, Djuro
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analysis
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Candidate biomarker
Carcinoma, Hepatocellular - metabolism
Cell Membrane - metabolism
Chromatography, High Pressure Liquid - methods
Fundamental and applied biological sciences. Psychology
General pharmacology
Hydrogen-Ion Concentration
Liver
Liver - metabolism
Liver Neoplasms, Experimental - metabolism
Mass spectrometry
Medical sciences
Molecular Sequence Data
Morris hepatoma 7777
Octoxynol - chemistry
Pharmacology. Drug treatments
Plasma membrane
Proteins - analysis
Proteins - chemistry
Proteins - isolation & purification
Proteomics - methods
Rats
Reproducibility of Results
Solubility
Spectrometry, Mass, Electrospray Ionization - methods
Tandem Mass Spectrometry - methods
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Summary:Plasma membranes from normal rat liver and hepatocellular carcinoma Morris hepatoma 7777 were selectively solubilized by use of different reagents. After selective solubilization, proteins were identified by nano-HPLC–electrospray ionization tandem mass spectrometry (LC-ESI MS/MS). Using simple software, the patterns of proteins identified in membrane solubilizates from liver and hepatoma were compared. Proteins identified in Morris hepatoma 7777 and not in the corresponding membrane solubilizate from liver, mostly members of the annexin and heat shock protein families, are discussed as potential candidate markers for hepatocellular carcinomas.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2006.08.047