HOMER2 binds MYO18B and enhances its activity to suppress anchorage independent growth

MYO18B is a class XVIII myosin, cloned as a tumor suppressor gene candidate. To investigate the mechanisms of MYO18B-dependent tumor suppression, MYO18B-interacting proteins were searched for by a yeast two-hybrid screen. HOMER2, a Homer/Ves1 family protein, was identified as a binding partner of MY...

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Published in:Biochemical and biophysical research communications 2007-05, Vol.356 (4), p.851-856
Main Authors: Ajima, Rieko, Kajiya, Keiko, Inoue, Takeshi, Tani, Masachika, Shiraishi-Yamaguchi, Yoko, Maeda, Masahiro, Segawa, Tatsuya, Furuichi, Teiichi, Sutoh, Kazuo, Yokota, Jun
Format: Article
Language:English
Subjects:
Actin binding protein
Anchorage-independent growth
Animals
Carrier Proteins - metabolism
Cell Adhesion - physiology
Cell Membrane - physiology
Cell Proliferation
Homer Scaffolding Proteins
HOMER2/Cupidin
Mice
Myosin
Myosins - metabolism
NIH 3T3 Cells
Protein Binding
Tumor Suppressor Proteins - metabolism
Yeast two-hybrid screen
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Summary:MYO18B is a class XVIII myosin, cloned as a tumor suppressor gene candidate. To investigate the mechanisms of MYO18B-dependent tumor suppression, MYO18B-interacting proteins were searched for by a yeast two-hybrid screen. HOMER2, a Homer/Ves1 family protein, was identified as a binding partner of MYO18B. These proteins co-localized in the regions of membrane protrusion and stress fiber, which are known as ones with filamentous actin-rich structures. Expression of HOMER2 enhanced the ability of MYO18B to suppress anchorage-independent growth. These results indicate that HOMER2 and MYO18B cooperate together in tumor suppression.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.03.060