Self-Sufficient Baeyer-Villiger Monooxygenases: Effective Coenzyme Regeneration for Biooxygenation by Fusion Engineering

Two‐in‐one biocatalysts were engineered by the covalent fusion of NADPH‐dependent Baeyer–Villiger monooxygenases to a phosphite dehydrogenase for coenzyme regeneration (see scheme). Not only the purified fusion proteins, but also whole cells and crude cell extracts containing the enzyme conjugates,...

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Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2008-03, Vol.47 (12), p.2275-2278
Main Authors: Torres Pazmiño, Daniel E, Snajdrova, Radka, Baas, Bert-Jan, Ghobrial, Michael, Mihovilovic, Marko D, Fraaije, Marco W
Format: Article
Language:English
Subjects:
asymmetric catalysis
Baeyer-Villiger reaction
biotransformations
coenzyme regeneration
Coenzymes - chemistry
Coenzymes - metabolism
fusion enzymes
Kinetics
Lactones - chemistry
Mixed Function Oxygenases - chemistry
Mixed Function Oxygenases - metabolism
Molecular Structure
Protein Engineering
Stereoisomerism
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Summary:Two‐in‐one biocatalysts were engineered by the covalent fusion of NADPH‐dependent Baeyer–Villiger monooxygenases to a phosphite dehydrogenase for coenzyme regeneration (see scheme). Not only the purified fusion proteins, but also whole cells and crude cell extracts containing the enzyme conjugates, could be used to catalyze biotransformations with high efficiency. NADP+=nicotinamide adenine dinucleotide phosphate.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200704630