A Polymerase Chain Reaction-Based Diagnostic to Identify Larvae and Eggs of Container Mosquito Species from the Australian Region

Dengue outbreaks occur regularly in parts of northern Queensland, Australia, and there is concern that these outbreaks may spread with the introduction and range expansion of the two main vectors Aedes aegypti (L.) and Aedes albopictus (Skuse). Problems encountered in separating larvae of endemic an...

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Bibliographic Details
Published in:Journal of medical entomology 2007-03, Vol.44 (2), p.376-380
Main Authors: Beebe, Nigel W., Whelan, Peter I., van den Hurk, Andrew F., Ritchie, Scott A., Corcoran, Sarah, Cooper, Robert D.
Format: Article
Language:English
Subjects:
Aedes - classification
Aedes - genetics
Aedes aegypti
Aedes albopictus
Aedes mosquitoes
Animals
Australia - epidemiology
Biological and medical sciences
container mosquito species
Dengue - epidemiology
dengue vectors
DNA - chemistry
DNA - isolation & purification
DNA Primers - chemistry
DNA, Ribosomal Spacer - genetics
Fundamental and applied biological sciences. Psychology
indigenous species
insect vectors
Insect Vectors - classification
Insect Vectors - genetics
internal transcribed spacer region 1
internal transcribed spacer-1
internal transcribed spacers
introduced species
Larva
larvae
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
molecular systematics
ova
Ovum
pest identification
polymerase chain reaction
Polymerase Chain Reaction - veterinary
polymerase chain reaction diagnostic
restriction fragment length polymorphism
ribosomal DNA
SHORT COMMUNICATIONS
Species Specificity
taxonomy
Time Factors
Vectors. Intermediate hosts
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Description
Summary:Dengue outbreaks occur regularly in parts of northern Queensland, Australia, and there is concern that these outbreaks may spread with the introduction and range expansion of the two main vectors Aedes aegypti (L.) and Aedes albopictus (Skuse). Problems encountered in separating larvae of endemic and exotic container mosquito species resulted in the development of a polymerase chain reaction diagnostic procedure that uses a restriction enzyme to cut the internal transcribed spacer region 1 of the ribosomal DNA to separate Ae. aegypti and Ae. albopictus from a number of common local container mosquito species which can be used at any stage of the life cycle, including eggs up to 8 wk of age. Identification was possible using desiccated or alcohol-preserved specimens with a response time of
ISSN:0022-2585
1938-2928
DOI:10.1603/0022-2585%282007%2944%5B376%3AAPCRDT%5D2.0.CO%3B2