Taxonomic and Phylogenetic Relationships Between Neotropical Species of Ticks from Genus Amblyomma (Acari: Ixodidae) Inferred from Second Internal Transcribed Spacer Sequences of rDNA

The accurate specific identification of ticks is essential for the study, control and prevention of tick-borne diseases. Herein, we determined ribosomal nucleotide sequences of the second internal transcribed spacer (ITS2) of 15 Neotropical hard tick species of the genus Amblyomma Koch found in Braz...

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Bibliographic Details
Published in:Journal of medical entomology 2007-03, Vol.44 (2), p.222-228
Main Authors: Marrelli, M. T., Souza, L. F., Marques, R. C., Labruna, M. B., Matioli, S. R., Tonon, A. P., Ribolla, P. E M., Marinotti, O., Schumaker, T. T S.
Format: Article
Language:English
Subjects:
Amblyomma
animal taxonomy
Animals
Arachnida
Base Sequence
Biological and medical sciences
Brazil
DNA Primers - chemistry
DNA, Ribosomal Spacer - chemistry
DNA, Ribosomal Spacer - genetics
Fundamental and applied biological sciences. Psychology
genetic markers
internal transcribed spacers
Invertebrates
ITS2
Ixodidae - classification
Ixodidae - genetics
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
Molecular Sequence Data
molecular systematics
nucleotide sequences
Phylogeny
Polymerase Chain Reaction
POPULATION BIOLOGY/GENETICS
ribosomal DNA
sequence analysis
Sequence Analysis, DNA - veterinary
sequence homology
Sequence Homology, Nucleic Acid
Species Specificity
taxonomy
tick identification
ticks
Vectors. Intermediate hosts
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Description
Summary:The accurate specific identification of ticks is essential for the study, control and prevention of tick-borne diseases. Herein, we determined ribosomal nucleotide sequences of the second internal transcribed spacer (ITS2) of 15 Neotropical hard tick species of the genus Amblyomma Koch found in Brazil. Most of the studied ticks accidentally parasite humans and potentially act as vectors of zoonoses. Lengths of the ITS2 sequences ranged from 956 to 1,207 bp, whereas GC content varied from 62.4 to 66.9%. A matrix of ITS2 divergence was calculated with the ITS2 sequence data obtained showing divergence levels varying from 1.5 to 28.8%. The analysis indicated that this molecular marker can be useful for Amblyomma-specific identification. Phylogenetic inferences based on the ITS2 sequences were used to assess some issues in subgenus taxonomy.
ISSN:0022-2585
1938-2928
DOI:10.1603/0022-2585%282007%2944%5B222%3ATAPRBN%5D2.0.CO%3B2