The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves

• Published in: Journal of ethnopharmacology 2008-03, Vol.116 (3), p.439-446
• Main Authors: Chumark, Pilaipark, Khunawat, Panya, Sanvarinda, Yupin, Phornchirasilp, Srichan, Morales, Noppawan Phumala, Phivthong-ngam, Laddawal, Ratanachamnong, Piyanee, Srisawat, Supath, Pongrapeeporn, Klai-upsorn S.
• Format: Article
• Language: English
• Subjects: animal models; Animals; Antiatherosclerotic; antiatherosclerotic activity; antioxidant activity; Antioxidants - pharmacology; aqueous solutions; Atherosclerosis - drug therapy; Atherosclerosis - prevention & control; Biological and medical sciences; Biphenyl Compounds; blood lipids; Body Weight - drug effects; cholesteremic effect; cholesterol; Cholesterol - blood; Cholesterol, Dietary - administration & dosage; Cholesterol, Dietary - pharmacology; dosage; dose response; free radical scavengers; free radicals; General pharmacology; Humans; Hypolipidaemic; Hypolipidemic Agents - pharmacology; in vitro studies; leaves; lipemic effect; lipid peroxidation; Lipids - blood; Lipoproteins, LDL - metabolism; low density lipoprotein; Male; Medical sciences; medicinal plants; medicinal properties; Moringa oleifera; Moringa oleifera - chemistry; Moringa oleifera Lam. leaves; Moringaceae; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Picrates - analysis; plant extracts; Plant Extracts - pharmacology; Plant Leaves - chemistry; Rabbits; Simvastatin - pharmacology; Thiobarbituric Acid Reactive Substances - analysis; Vitamin E - pharmacology; Water - chemistry
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2007.12.010

Moringa oleifera is used in Thai traditional medicine as cardiotonic. Recent studies demonstrated its hypocholesterolaemic effect. However, to be clinically useful, more scientific data are needed. Aim of the Study: We investigated the antioxidant, hypolipidaemic and antiatherosclerotic activities of Moringa oleifera leaf extract. Materials and Methods: Scavenging activity of the extract on 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH), and the inhibitory effect on Cu 2+-induced low-density lipoprotein (LDL) oxidation were determined in in vitro experiment. The effects of the extract on cholesterol levels, conjugated diene (CD) and thiobarbituric acid reactive substances (TBARS) and plaque formations in cholesterol-fed rabbits were investigated. Results: We found that in scavenging DPPH radicals the extract and Trolox ® had IC 50 of 78.15 ± 0.92 and 2.14 ± 0.12 μg/ml, respectively. The extract significantly ( P < 0.05) prolonged the lag-time of CD formation and inhibited TBARS formation in both in vitro and ex vivo experiments in a dose-dependent manner. In hypercholesterol-fed rabbits, at 12 weeks of treatment, it significantly ( P < 0.05) lowered the cholesterol levels and reduced the atherosclerotic plaque formation to about 50 and 86%, respectively. These effects were at degrees comparable to those of simvastatin. Conclusions: The results indicate that this plant possesses antioxidant, hypolipidaemic and antiatherosclerotic activities and has therapeutic potential for the prevention of cardiovascular diseases.