An Immunonanogold Resonance Scattering-Quenching Probe for Rapid and Sensitive Assay of Microalbumin

• Published in: Journal of fluorescence 2008-03, Vol.18 (2), p.563-571
• Main Authors: Jiang, Zhi-Liang, Huang, Yu-Juan, Liang, Ai-Hui
• Format: Article
• Language: English
• Subjects: Albumins - analysis; Analytical Chemistry; Biochemistry; Biological and Medical Physics; Biological Assay; Biomedical and Life Sciences; Biomedicine; Biophysics; Biotechnology; Fluoroimmunoassay - methods; Fluoroimmunoassay - standards; Gold Colloid - chemistry; Humans; Metal Nanoparticles - analysis; Metal Nanoparticles - chemistry; Original Paper; Scattering, Radiation; Sensitivity and Specificity
• ISSN: 1053-0509; 1573-4994
• DOI: 10.1007/s10895-007-0300-3

A novel and sensitive immunonanogold resonance scattering (RS) spectral probe was obtained for rapid detection of microalbumin (Malb), using 10 nm gold nanaoparticle to label goat anti-human Malb. It was based on that the gold-labeled anti-Malb took place nonspecific aggregation and exhibited a strong RS peak at 577 nm in pH 5.2 C 6 H 8 O 7 –Na 2 HPO 4 buffer solution containing polyethylene glycol (PEG), and the immunocomplex formed after specific reaction of gold-labeled anti-Malb with Malb, which led to a decrease in the intensity of RS peak at 577 nm considerably. The decreased RS intensity at 577 nm (Δ I 577nm ) was linear to the concentration of Malb in the range of 4–128 ng/mL, with a detection limit of 3.2 ng/mL. The proposed method was applied to detect Malb in healthy human urine samples with satisfactory results.