Evaluation of non-specific immune components from the skin mucus of olive flounder ( Paralichthys olivaceus)

The innate immune system, particularly the external body surface, plays a frontier role in protecting fish under intensive aquaculture and at prolonged low temperatures from relevant infections due to inadequate adaptive immune responses. In the present study we aimed to understand the mucosal immun...

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Bibliographic Details
Published in:Fish & shellfish immunology 2008-04, Vol.24 (4), p.479-488
Main Authors: Palaksha, K.J., Shin, Gee-Wook, Kim, Young-Rim, Jung, Tae-Sung
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Animals
Antibacterial agglutinin
Bacteria - growth & development
Bacteria - immunology
Caseins - metabolism
Enzyme-Linked Immunosorbent Assay
Esterase
Esterases - metabolism
Flounder - immunology
Hydrolysis
Immunity, Innate - immunology
Immunoblotting
Immunoglobulin M
Immunoglobulin M - immunology
Lysozyme
Marine
Microbial Sensitivity Tests
Mucus - chemistry
Mucus - immunology
Muramidase - metabolism
Paralichthys olivaceus
Pleuronectiformes
Protease
Skin - chemistry
Skin - immunology
Skin mucus
Transferrin
Transferrin - metabolism
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Summary:The innate immune system, particularly the external body surface, plays a frontier role in protecting fish under intensive aquaculture and at prolonged low temperatures from relevant infections due to inadequate adaptive immune responses. In the present study we aimed to understand the mucosal immunity of an economically important mariculture fish, olive flounder ( Paralichthys olivaceus) by evaluating the immune components from its skin mucus. The activities of lysozyme (233.33 ± 171.82 units mg −1), trypsin-like protease (42.84 ± 1.249 units mg −1), alkaline phosphatase (0.376 ± 0.005 units mg −1) and esterase (0.170 ± 0.006 units mg −1) were detected in the skin mucus. Transferrin was identified by MALDI-TOF/MS analysis. ELISA and immunoblot assays using anti-flounder IgM monoclonal antibody showed the presence of a significant level (1.80 ± 0.001, n = 3) of monomer immunoglobulin M (IgM) with approximate molecular weight of 160 and 25 kDa under non-denaturing and denaturing states, respectively. Skin mucus showed strong antibacterial activity against tested fish pathogenic bacteria. In addition, skin mucus successfully agglutinated (HA titre 2 8), but completely failed to haemolyse, rabbit erythrocytes. In conclusion, the major immune components of the skin mucus, identified in the present study, are possibly involved in the broad spectrum non-specific immunity of olive flounder.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2008.01.005