DNA Metastability and Biological Regulation:  Conformational Dynamics of Metastable Ω-DNA Bulge Loops

Dynamic interchange between DNA conformations, including metastable states, can be of importance to biological function. In this study, we use a combination of spectroscopic and calorimetric techniques to detect and characterize kinetically trapped, metastable states in strand exchange and strand di...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2007-04, Vol.129 (16), p.5272-5280
Main Authors: Völker, Jens, Klump, Horst H, Breslauer, Kenneth J
Format: Article
Language:English
Subjects:
Base Sequence
DNA - chemistry
DNA - genetics
DNA - radiation effects
Gene Expression Regulation
Molecular Sequence Data
Mutation
Nucleic Acid Conformation - radiation effects
Oligodeoxyribonucleotides - chemistry
Oligodeoxyribonucleotides - genetics
Oligodeoxyribonucleotides - radiation effects
Spectrum Analysis
Temperature
Thermodynamics
Ultraviolet Rays
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Summary:Dynamic interchange between DNA conformations, including metastable states, can be of importance to biological function. In this study, we use a combination of spectroscopic and calorimetric techniques to detect and characterize kinetically trapped, metastable states in strand exchange and strand displacement reactions for bulge loop DNA conformations, here referred to as Ω-DNAs. We show that such metastable, Ω-DNA bulge loop states can stably coexist below 50 °C, while rearranging irreversibly at elevated temperatures to thermodynamically more stable states. Such dynamic interchange between metastable and globally stable DNA conformational states can be of importance in biological regulatory mechanisms.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja070258q