An electrochemical approach for detection of specific DNA-binding protein by gold nanoparticle-catalyzed silver enhancement

Interaction between transcription factor and sequence-specific DNA plays an important role in regulation of gene transcription in biological systems. As electrochemical intercalators, gold (Au) nanoparticles show high catalysis activity and compatibility for detection of biological molecules. In thi...

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Bibliographic Details
Published in:Analytical biochemistry 2008-04, Vol.375 (2), p.179-186
Main Authors: Pan, Qin, Zhang, Renyun, Bai, Yunfei, He, Nongyue, Lu, Zuhong
Format: Article
Language:English
Subjects:
Base Sequence
Binding Sites
Catalysis
DNA - genetics
DNA - metabolism
DNA-Binding Proteins - analysis
DNA-Binding Proteins - metabolism
Electrochemistry
Electrodes
Gold (Au) nanoparticle
Gold - chemistry
Gold - metabolism
Guanine
Kinetics
Metal Nanoparticles - chemistry
Molecular Sequence Data
NF-kappa B - metabolism
NF–κB
Oligonucleotides - genetics
Oligonucleotides - metabolism
Silver (Ag) enhancement
Silver - chemistry
Substrate Specificity
Transcription factor
Unimolecular hairpin oligonucleotide
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Summary:Interaction between transcription factor and sequence-specific DNA plays an important role in regulation of gene transcription in biological systems. As electrochemical intercalators, gold (Au) nanoparticles show high catalysis activity and compatibility for detection of biological molecules. In this article, we report an electrochemical approach for sequence-specific DNA-binding transcription factor detection by Au nanoparticle-catalyzed silver (Ag) enhancement at interface between electrodes and electrolyte solutions. Here unimolecular hairpin oligonucleotides were self-assembled onto Au electrode surface and their elongation on Au electrode surface was carried out to form double-stranded oligonucleotides with transcription factor NF-κB (nuclear factor–kappa B) binding sites. Au nanoparticle-catalyzed Ag deposition was detected by anodic stripping voltammetry (ASV) for NF-κB binding. It was found that this method for the detection of sequence-specific DNA-binding protein showed pronounced specificity and that the detection limit was as low as 0.1 pM. The findings indicated that our method can have applications in transcription regulation, operator site recognition, and functional gene inspection.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2007.12.006