Diabetic rats supplemented with L-glutamine : A study of immunoreactive myosin-V myenteric neurons and the proximal colonic mucosa

• Published in: Digestive diseases and sciences 2007-05, Vol.52 (5), p.1233-1241
• Main Authors: TASHIMA, Cristiano Massao, TRONCHINI, Eleandro Aparecido, PEREIRA, Renata Virginia Fernandes, BAZOTTE, Roberto Barbosa, ZANONI, Jacqueline Nelisis
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - administration & dosage; Antioxidants - pharmacology; Biological and medical sciences; Cell Proliferation - drug effects; Colon - drug effects; Colon - innervation; Colon - metabolism; Diabetes; Diabetes Mellitus, Experimental - complications; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - metabolism; Diabetes Mellitus, Experimental - pathology; Diabetes. Impaired glucose tolerance; Diabetic Neuropathies - etiology; Diabetic Neuropathies - metabolism; Diabetic Neuropathies - pathology; Diabetic Neuropathies - prevention & control; Diet; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Glutamine - administration & dosage; Glutamine - pharmacology; Immunohistochemistry; Intestinal Mucosa - drug effects; Intestinal Mucosa - innervation; Intestinal Mucosa - metabolism; Male; Medical sciences; Myenteric Plexus - drug effects; Myenteric Plexus - metabolism; Myenteric Plexus - pathology; Myosin Type V - metabolism; Neurons - drug effects; Neurons - metabolism; Oxidative stress; Rats; Rats, Wistar; Rodents; Time Factors; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-006-9564-8

We studied the neuronal density and size of myenteric neurons and the epithelial cell proliferation and crypt depth of the proximal colon in diabetic Wistar rats after supplementing them with L-glutamine (1%). The animals were divided into five groups: untreated normoglycemic (UN), L-glutamine-treated normoglycemic (NG), untreated diabetic (UD), and L-glutamine-treated diabetics 4 days (DG4) and 45 days (DG45) days after the onset of diabetes. We observed a reduction of 52.7% and 50.44% in the neuronal density of the proximal colon of the UD group compared to the UN and NG groups, respectively (P0.05). There were no significant differences (P>0.05) when the data relative to the area of the myenteric neuron cell bodies, metaphasic index, and crypt depth in the proximal colon were compared among experimental groups.