Prawn lipocalin: Characteristics and expressional pattern in subepidermal adipose tissue during reproductive molting cycle

In crustaceans, the fascinating processes of maturation, reproductive molting and carapace coloration are regulated by hydrophobic molecules. Interestingly, most of the molecules are ligands of lipocalin. To understand the role of lipocalin in the aforementioned processes at molecular level, we isol...

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Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2007-06, Vol.147 (2), p.222-229
Main Authors: Wang, Mo-Ran, Zhu, Xiao-Jing, Yang, Jin-Shu, Dai, Zhong-Min, Mahmood, Khalid, Yang, Fan, Yang, Wei-Jun
Format: Article
Language:English
Subjects:
Adipose Tissue - metabolism
Amino Acid Sequence
Animals
Base Sequence
Blotting, Southern
cDNA
DNA Primers
DNA, Complementary - genetics
Freshwater
Lipocalin
Lipocalins - genetics
Lipocalins - metabolism
Macrobrachium rosenbergii
Maturation
Models, Molecular
Molecular Sequence Data
Molting - genetics
Molting - physiology
Molting cycle
Penaeidae - genetics
Penaeidae - metabolism
Prawn
Sequence Analysis, DNA
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Summary:In crustaceans, the fascinating processes of maturation, reproductive molting and carapace coloration are regulated by hydrophobic molecules. Interestingly, most of the molecules are ligands of lipocalin. To understand the role of lipocalin in the aforementioned processes at molecular level, we isolated a cDNA that belongs to the lipocalin family, from a central nervous system cDNA library of Macrobrachium rosenbergii. We monitored the spatial and temporal distributions of the mRNA by using Northern Blotting analysis. Our results demonstrated that this gene expresses abundantly in the subepidermal adipose tissue, while faintly in the hepatopancreas and central nervous system. However, no signal was detected in other tissues including muscle, gill and ovary. Its expression levels in subepidermal adipose tissue during various stages of maturation as well as through the whole molting cycle showed that prawn lipocalin is involved in sexual maturation, as the maximal level was observed just after molt.
ISSN:1096-4959
1879-1107
DOI:10.1016/j.cbpb.2007.01.003