Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis

Novel fluoroalkyl chloroformates with three and four carbon atoms were investigated for the immediate conversion of amino acids into hydrophobic derivatives in water-containing media. Derivatization conditions were extensively studied and optimized sample preparation protocols elaborated. More than...

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Bibliographic Details
Published in:Journal of Chromatography A 2008-04, Vol.1186 (1), p.391-400
Main Authors: Hušek, Petr, Šimek, Petr, Hartvich, Petr, Zahradníčková, Helena
Format: Article
Language:English
Subjects:
Alcohols
Amino acids
Amino Acids - analysis
Amino Acids - chemistry
Catalysis
Derivatization
Flame Ionization
Formates - chemistry
Gas chromatography
Gas Chromatography-Mass Spectrometry - methods
Pentafluoropropyl- and heptafluorobutyl chloroformates
Picolines - chemistry
Pyridines - chemistry
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Summary:Novel fluoroalkyl chloroformates with three and four carbon atoms were investigated for the immediate conversion of amino acids into hydrophobic derivatives in water-containing media. Derivatization conditions were extensively studied and optimized sample preparation protocols elaborated. More than 30 amino acids were treated with the particular reagent in isooctane by simply vortexing the reactive organic phase with a slightly basified aqueous medium containing pyridine or 3-picoline as a catalyst. Outstanding separation of nearly all components on 5% phenylmethylsilicone phase in gas chromatographic (GC) analysis with mass spectrometric (MS) or flame ionization detection (FID) required
ISSN:0021-9673
DOI:10.1016/j.chroma.2007.11.117