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Activation of Complement Component C5: COMPARISON OF C5 CONVERTASES OF THE LECTIN PATHWAY AND THE CLASSICAL PATHWAY OF COMPLEMENT

Although the initiating complex of lectin pathway (called M1 in this study) generates C3/C5 convertases similar to those assembled by the initiating complex (C1) of the classical pathway, activation of complement component C5 via the lectin pathway has not been examined. In the present study kinetic...

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Published in:The Journal of biological chemistry 2008-03, Vol.283 (12), p.7853-7863
Main Authors: Rawal, Nenoo, Rajagopalan, Rema, Salvi, Veena P
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Rajagopalan, Rema
Salvi, Veena P
description Although the initiating complex of lectin pathway (called M1 in this study) generates C3/C5 convertases similar to those assembled by the initiating complex (C1) of the classical pathway, activation of complement component C5 via the lectin pathway has not been examined. In the present study kinetic analysis of lectin pathway C3/C5 convertases assembled on two surfaces (zymosan and sheep erythrocytes coated with mannan (EMan)) revealed that the convertases (ZymM1,C4b,C2a and EManM1,C4b,C2a) exhibited a similar but weak affinity for the substrate, C5 indicated by a high Km (2.73-6.88 μM). Very high affinity C5 convertases were generated when the low affinity C3/C5 convertases were allowed to deposit C3b by cleaving native C3. These C3b-containing convertases exhibited Km (0.0086-0.0075 μM) well below the normal concentration of C5 in blood (0.37 μM). Although kinetic parameters, Km and kcat, of the lectin pathway C3/C5 convertases were similar to those reported for classical pathway C3/C5 convertases, studies on the ability of C4b to bind C2 indicated that every C4b deposited on zymosan or EMan was capable of forming a convertase. These findings differ from those reported for the classical pathway C3/C5 convertase, where only one of four C4b molecules deposited formed a convertase. The potential for four times more amplification via the lectin pathway than the classical pathway in the generation of C3/C5 convertases and production of pro-inflammatory products, such as C3a, C4a, and C5a, implies that activation of complement via the lectin pathway might be a more prominent contributor to the pathology of inflammatory reactions.
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subjects Animals
Chickens
Complement C2 - metabolism
Complement C3-C5 Convertases - metabolism
Complement C3b - metabolism
Complement C4b - metabolism
Complement C5 - metabolism
Complement Pathway, Classical - drug effects
Complement Pathway, Classical - physiology
Complement Pathway, Mannose-Binding Lectin - drug effects
Complement Pathway, Mannose-Binding Lectin - physiology
Erythrocytes - enzymology
Humans
Inflammation - metabolism
Inflammation Mediators - metabolism
Protein Binding - drug effects
Protein Binding - physiology
Sheep
Zymosan - pharmacology
title Activation of Complement Component C5: COMPARISON OF C5 CONVERTASES OF THE LECTIN PATHWAY AND THE CLASSICAL PATHWAY OF COMPLEMENT
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