Differential platelet-activating factor synthesis by monocytes and polymorphonuclear leukocytes from subjects with localized aggressive periodontitis

Background and Objective:  Platelet‐activating factor is elevated in localized aggressive periodontitis. We previously demonstrated that the elevated level of platelet‐activating factor in localized aggressive periodontitis is at least partially attributable to low levels of platelet‐activating fact...

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Published in:Journal of periodontal research 2007-06, Vol.42 (3), p.202-211
Main Authors: Shin, C. R., Moores, J., Best, A. M., Tew, J. G., Schenkein, H. A., Barbour, S. E.
Format: Article
Language:English
Subjects:
Adult
Analysis of Variance
Animals
Biological and medical sciences
Calcimycin - metabolism
Case-Control Studies
Cattle
Confidence Intervals
Dentistry
Facial bones, jaws, teeth, parodontium: diseases, semeiology
Humans
Medical sciences
monocyte
Monocytes - metabolism
neutrophil
Neutrophils - metabolism
Non tumoral diseases
Otorhinolaryngology. Stomatology
periodontitis
Periodontitis - metabolism
Platelet Activating Factor - analysis
Platelet Activating Factor - biosynthesis
platelet-activating factor
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Summary:Background and Objective:  Platelet‐activating factor is elevated in localized aggressive periodontitis. We previously demonstrated that the elevated level of platelet‐activating factor in localized aggressive periodontitis is at least partially attributable to low levels of platelet‐activating factor acetylhydrolase, the enzyme that catabolizes platelet‐activating factor. The objective of this study was to determine if platelet‐activating factor synthesis was also elevated in localized aggressive periodontitis. To test this, platelet‐activating factor synthesis was quantified in the monocytes and polymorphonuclear neutrophils of periodontally healthy patients and of subjects with localized aggressive periodontitis. Material and Methods:  Cells were labeled with [3H]acetate and treated with vehicle or stimulated with calcium ionophore A23187. Platelet‐activating factor was extracted and quantified by scintillation counting. Results:  For both subject groups, resting monocytes and polymorphonuclear neutrophils produced platelet‐activating factor, and calcium ionophore A23187 stimulated platelet‐activating factor production in both cell types. However, calcium ionophore A23187‐activated monocytes from subjects with localized aggressive periodontitis produced less platelet‐activating factor than did activated periodontally healthy monocytes (p 
ISSN:0022-3484
1600-0765
DOI:10.1111/j.1600-0765.2006.00933.x