Cohesin Complex Promotes Transcriptional Termination between Convergent Genes in S. pombe

Transcription analyses reported in these studies reveal that convergent genes in S. pombe generate overlapping transcripts in the G1 phase of the cell cycle. We show that this double-strand (ds) RNA induces localized RNAi (Dicer and RITS) dependent transient heterochromatin structures including hist...

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Bibliographic Details
Published in:Cell 2008-03, Vol.132 (6), p.983-995
Main Authors: Gullerova, Monika, Proudfoot, Nick J.
Format: Article
Language:English
Subjects:
Cell Cycle Proteins - metabolism
CELLCYCLE
Chromosomal Proteins, Non-Histone - metabolism
Codon, Terminator
Cohesins
DNA
G1 Phase
G2 Phase
Gene Expression Regulation, Fungal
Heterochromatin
Mitosis
Nuclear Proteins - metabolism
RNA
RNA Interference
RNA, Double-Stranded - metabolism
RNA, Messenger
Schizosaccharomyces - cytology
Schizosaccharomyces - genetics
Schizosaccharomyces pombe Proteins - metabolism
Transcription, Genetic
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Summary:Transcription analyses reported in these studies reveal that convergent genes in S. pombe generate overlapping transcripts in the G1 phase of the cell cycle. We show that this double-strand (ds) RNA induces localized RNAi (Dicer and RITS) dependent transient heterochromatin structures including histone H3 lysine 9 trimethylation marks and Swi6 association. Consequently cohesin is recruited to these chromosomal positions through interaction with Swi6. In G2, localized cohesin is further concentrated into the intergenic regions of the convergent genes tested. This results in a block to further dsRNA formation by promoting gene-proximal transcription termination between the convergent genes. Cohesin release at mitosis leads to a new G1 phase with repeated dsRNA formation, transient heterochromatin, and cohesin recruitment. Our results uncover a hitherto unanticipated role for cohesin and further suggest a widespread role for the selective formation of dsRNA, heterochromatin, and subsequent cohesin recruitment in regulated transcriptional termination.
ISSN:0092-8674
1097-4172
DOI:10.1016/j.cell.2008.02.040