Investigation on the infection mechanism of the fungus Clonostachys rosea against nematodes using the green fluorescent protein

The fungus Clonostachys rosea (syn. Gliocladium roseum) is a potential biocontrol agent. It can suppress the sporulation of the plant pathogenic fungus Botrytis cinerea and kill pathogenic nematodes, but the process of nematode pathogenesis is poorly understood. To help understand the underlying mec...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2008-04, Vol.78 (6), p.983-990
Main Authors: Zhang, Lin, Yang, Jinkui, Niu, Qiuhong, Zhao, Xuna, Ye, Fengping, Liang, Lianming, Zhang, Ke-Qin
Format: Article
Language:English
Subjects:
Animals
Applied Genetics and Molecular Biotechnology
Biological and medical sciences
Biological control
Biotechnology
Blotting, Southern
Botrytis cinerea
Clonostachys rosea
Clonostachys rosea f. rosea
Fluorescence microscopy
Fundamental and applied biological sciences. Psychology
Fungi
Genomes
Gliocladium roseum
Green fluorescent protein (GFP)
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Hypocreales - genetics
Hypocreales - physiology
infection
Infections
Laboratories
Life Sciences
Microbial Genetics and Genomics
Microbiology
Nematoda
Nematoda - microbiology
Nematoda - physiology
Nematodes
Pathogenesis
Pesticides
Plasmids
Plasmids - genetics
Plasmids - metabolism
Proteins
Protoplast transformation
Studies
Transformation, Genetic
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Summary:The fungus Clonostachys rosea (syn. Gliocladium roseum) is a potential biocontrol agent. It can suppress the sporulation of the plant pathogenic fungus Botrytis cinerea and kill pathogenic nematodes, but the process of nematode pathogenesis is poorly understood. To help understand the underlying mechanism, we constructed recombinant strains containing a plasmid with both the enhanced green fluorescent protein gene egfp and the hygromycin resistance gene hph. Expression of the green fluorescent protein (GFP) was monitored using fluorescence microscopy. Our observations reveal that the pathogenesis started from the adherence of conidia to nematode cuticle for germination, followed by the penetration of germ tubes into the nematode body and subsequent death and degradation of the nematodes. These are the first findings on the infection process of the fungal pathogen marked with GFP, and the developed method can become an important tool for studying the molecular mechanisms of nematode infection by C. rosea.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-008-1392-7