Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry

• Published in: Biomedical chromatography 2008-04, Vol.22 (4), p.414-420
• Main Authors: Li, Zhang, Hua, Yu, Ming, Li Wen, Chun, Cheung Man, Ping, Pang Yuan, Ge, Lin, Tao, Wang Yi, Zhong, Zuo, Fan, Han Yi
• Format: Article
• Language: English
• Subjects: Alzheimer's disease; Animals; bis-tacrine; Cholinesterase Inhibitors - blood; Cholinesterase Inhibitors - chemistry; Cholinesterase Inhibitors - metabolism; Chromatography, High Pressure Liquid - methods; Erythrocytes - metabolism; LC-MS-MS; Molecular Structure; rat plasma; Rats; Reproducibility of Results; Tacrine - blood; Tacrine - chemistry; Tacrine - metabolism; Tandem Mass Spectrometry - methods
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.949

The current study aims to develop a specific and sensitive LC‐MS/MS method for determination of bis(7)‐tacrine (B7T) in rat plasma. A 100 µL plasma sample was extracted with ethyl acetate. B7T and the internal standard (IS), pimozide, in the samples were then analyzed with LC‐MS/MS in positive electrospray ionization condition. Chromatographic separation of B7T and IS was achieved in a C18 reversed‐phase HPLC column (150 × 2.1 mm i.d.) by isocratic elution with a mobile phase consisting of 0.05% formic acid in water and acetonitrile (1:1, v/v) at a flow rate of 0.35 mL/min. Multiple‐reaction monitoring (MRM) mode was employed to measure the ion transitions: m/z 247 to 197 for B7T and m/z 462 to m/z 328 for IS, respectively. The method was linear over the studied ranges of 100–5000 and 10–100 ng/mL. The intra‐day and inter‐day variations of the analysis were less than 6.8% with standard errors less than 9.0%. The detection limit of B7T in rat plasma was 1 ng/mL. The developed method was successfully applied to the pharmacokinetic study of B7T after intravenous administration of 1 mg/kg B7T and further proved to be readily utilized for determination of B7T in rat plasma samples. Copyright © 2007 John Wiley & Sons, Ltd.