Available (Ileal Digestible Reactive) Lysine in Selected Pet Foods

A recently developed assay for determining available lysine (true ileal digestible reactive lysine) in foods and feedstuffs was applied to 20 commercially available cat foods. Semisynthetic diets, containing cat food as the sole protein source, were prepared. Titanium dioxide was included as an indi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 2007-05, Vol.55 (9), p.3517-3522
Main Authors: Rutherfurd, Shane M, Rutherfurd-Markwick, Kay J, Moughan, Paul J
Format: Article
Language:English
Subjects:
Animals
bioavailability
Biological and medical sciences
Biological Availability
cat foods
Cats
digestibility
Digestion
Food Analysis
food composition
Food industries
Fundamental and applied biological sciences. Psychology
ileum
Ileum - metabolism
intestinal absorption
lysine
Lysine - analysis
nutrient availability
pet foods
rats
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A recently developed assay for determining available lysine (true ileal digestible reactive lysine) in foods and feedstuffs was applied to 20 commercially available cat foods. Semisynthetic diets, containing cat food as the sole protein source, were prepared. Titanium dioxide was included as an indigestible marker. The diets were fed to growing rats and digesta from the terminal ileum collected and analyzed, along with the diets, for reactive lysine. True reactive lysine digestibility was determined after correction for endogenous lysine loss at the terminal ileum of rats fed an enzyme-hydrolyzed casein-based diet. The amounts of digestible total lysine (conventional method) were also determined. Ileal total lysine digestibility significantly (P < 0.05) underestimated (3.6−10.2%) lysine availability (ileal reactive lysine digestibility) for most of the cat foods tested. Ileal digestible total lysine significantly (P < 0.05) overestimated the amount of dietary available lysine for all of the cat foods tested by between 38 and 143%. Total lysine digestibility determined using the conventional method of lysine analysis was inaccurate when applied to commercially available cat foods. Keywords: Lysine; availability; cat food; processing
ISSN:0021-8561
1520-5118
DOI:10.1021/jf062919t