Metallo-β-lactamase VIM-2 in Pseudomonas aeruginosa isolates from a cystic fibrosis patient

Abstract The increased incidence of Pseudomonas aeruginosa isolated from patients with cystic fibrosis (CF) along with an increase in its multidrug resistance makes therapeutic management very problematic. Careful identification and accurate studies of susceptibility to antibiotics are critical for...

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Bibliographic Details
Published in:International journal of antimicrobial agents 2008-04, Vol.31 (4), p.375-379
Main Authors: Cardoso, Olga, Alves, Ana Florinda, Leitão, Rui
Format: Article
Language:English
Subjects:
Adolescent
Antibiotics. Antiinfectious agents. Antiparasitic agents
beta-Lactamases - biosynthesis
beta-Lactamases - metabolism
Biological and medical sciences
Child
Child, Preschool
Cystic fibrosis
Cystic Fibrosis - microbiology
DNA, Bacterial - genetics
Errors of metabolism
Humans
Infant
Infectious Disease
Medical sciences
Metabolic diseases
Metallo-β-lactamase
Microbial Sensitivity Tests
Miscellaneous hereditary metabolic disorders
Pharmacology. Drug treatments
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - enzymology
Reverse Transcriptase Polymerase Chain Reaction
Surveillance
VIM-2
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Summary:Abstract The increased incidence of Pseudomonas aeruginosa isolated from patients with cystic fibrosis (CF) along with an increase in its multidrug resistance makes therapeutic management very problematic. Careful identification and accurate studies of susceptibility to antibiotics are critical for improving therapeutic measures and for facilitating our understanding of the epidemiology of this pathogen. Fifteen P. aeruginosa isolates obtained from five CF children in the Paediatric Hospital of Coimbra were studied. Isolates from a female patient were resistant to all agents tested except colistin. A VIM-2 enzyme inserted in integron In 58 was detected, and this isolate presented a unique random amplified polymorphic DNA (RAPD) type. Others isolates were susceptible to β-lactams, and each isolate had a different RAPD type. VIM-2 confers resistance to the majority of β-lactams and is associated with other gene cassettes coding for enzymes that inactivate aminoglycosides. Person-to-person transmission of these isolates is not well understood, therefore it is important to design infection control policies to avoid acquisition and dissemination of multiresistant strains.
ISSN:0924-8579
1872-7913
DOI:10.1016/j.ijantimicag.2007.12.006