Identification and classification of respiratory syncytial virus (RSV) strains by surface-enhanced Raman spectroscopy and multivariate statistical techniques

There is a critical need for a rapid and sensitive means of detecting viruses. Recent reports from our laboratory have shown that surface-enhanced Raman spectroscopy (SERS) can meet these needs. In this study, SERS was used to obtain the Raman spectra of respiratory syncytial virus (RSV) strains A/L...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2008-03, Vol.390 (6), p.1551-1555
Main Authors: Shanmukh, S, Jones, L, Zhao, Y.-P, Driskell, J. D, Tripp, R. A, Dluhy, R. A
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biochemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Cluster Analysis
detection
Food Science
Laboratory Medicine
Monitoring/Environmental Analysis
Multivariate Analysis
Multivariate statistics
nanorods
Respiratory Syncytial Viruses - chemistry
Respiratory Syncytial Viruses - classification
RSV
SERS
Short Communication
Spectrum Analysis, Raman - methods
Surface Properties
viruses
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Summary:There is a critical need for a rapid and sensitive means of detecting viruses. Recent reports from our laboratory have shown that surface-enhanced Raman spectroscopy (SERS) can meet these needs. In this study, SERS was used to obtain the Raman spectra of respiratory syncytial virus (RSV) strains A/Long, B1, and A2. SERS-active substrates composed of silver nanorods were fabricated using an oblique angle vapor deposition method. The SERS spectra obtained for each virus were shown to posses a high degree of reproducibility. Based on their intrinsic SERS spectra, the four virus strains were readily detected and classified using the multivariate statistical methods principal component analysis (PCA) and hierarchical cluster analysis (HCA). The chemometric results show that PCA is able to separate the three virus strains unambiguously, whereas the HCA method was able to readily distinguish an A2 strain-related G gene mutant virus (ΔG) from the A2 strain. The results described here demonstrate that SERS, in combination with multivariate statistical methods, can be utilized as a highly sensitive and rapid viral identification and classification method.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-008-1851-0