Differential expression of two vasa/PL10-related genes during gametogenesis in the special model system Rana

Germline cell fate decisions are primarily controlled at the post-transcriptional level with DEAD-box RNA helicases playing a crucial role in germline development. In this study, we report the identification of two DEAD-box vasa/PL10 orthologues (RlVlg and RlPL10) in a species complex of the genus R...

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Bibliographic Details
Published in:Development genes and evolution 2007-05, Vol.217 (5), p.395-402
Main Authors: Marracci, Silvia, Casola, Claudio, Bucci, Stefania, Ragghianti, Matilde, Ogielska, Maria, Mancino, Giorgio
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cell fate
DEAD-box RNA Helicases - chemistry
DEAD-box RNA Helicases - genetics
DEAD-box RNA Helicases - metabolism
Endoreduplication
Gametogenesis
Gametogenesis - genetics
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genomes
germ cells
Hybridogenesis
Male
Meiosis
Molecular Sequence Data
Oogenesis - genetics
Post-transcription
Rana esculenta complex
Ranidae - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Spermatogenesis - genetics
Testis - cytology
Testis - metabolism
Vasa and PL10 genes
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Summary:Germline cell fate decisions are primarily controlled at the post-transcriptional level with DEAD-box RNA helicases playing a crucial role in germline development. In this study, we report the identification of two DEAD-box vasa/PL10 orthologues (RlVlg and RlPL10) in a species complex of the genus Rana, characterized by hybridogenetic reproduction, an enigmatic process that involves the exclusion of an individual genome, and endoreduplication events. Both genes were expressed during the early stages of gametogenesis of R. ridibunda, R. lessonae, and their natural hybrid R. esculenta. RlVlg expression was germline specific. On the other hand, RlPL10 was also expressed in somatic tissues, although only at low levels. The two genes were expressed in different phases of mitotic and meiotic spermatogenetic divisions as demonstrated by immunostaining with an anti-H3 phosphohistone antibody. The data indicate that RlVlg and RlPL10 may represent useful markers for dissecting the molecular aspects of genome exclusion and endoreduplication of the hybridogenetic gametogenesis.
ISSN:0949-944X
1432-041X
DOI:10.1007/s00427-007-0143-6