Different pattern of pleiotrophin and midkine expression in neuropathic pain: Correlation between changes in pleiotrophin gene expression and rat strain differences in neuropathic pain

Pleiotrophin (PTN) and midkine (MK) are two growth factors highly redundant in function that exhibit neurotrophic actions and are upregulated at sites of nerve injury, both properties being compatible with a potential involvement in the pathophysiological events that follow nerve damage (i.e. neurop...

Full description

Saved in:
Bibliographic Details
Published in:Growth factors (Chur, Switzerland) Switzerland), 2008-02, Vol.26 (1), p.44-48
Main Authors: Ezquerra, Laura, Alguacil, Luis F., Nguyen, Trang, Deuel, Thomas F., Silos-Santiago, Inmaculada, Herradon, Gonzalo
Format: Article
Language:English
Subjects:
Animals
Carrier Proteins - biosynthesis
Cytokines - biosynthesis
Ganglia, Spinal - pathology
Gene Expression Regulation
mechanical allodynia
midkine
Neurons - metabolism
Pain
Peripheral Nerve Injuries
Peripheral nerve injury
pleiotrophin
Rats
Rats, Inbred F344
Rats, Inbred Lew
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Species Specificity
Up-Regulation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pleiotrophin (PTN) and midkine (MK) are two growth factors highly redundant in function that exhibit neurotrophic actions and are upregulated at sites of nerve injury, both properties being compatible with a potential involvement in the pathophysiological events that follow nerve damage (i.e. neuropathic pain). We have tested this hypothesis by comparatively studying PTN and MK gene expression in the spinal cord and dorsal root ganglia (DRG) of three rat strains known to differ in their behavioural responses to chronic constriction injury (CCI) of the sciatic nerve: Lewis, Fischer 344 (F344) and Sprague-Dawley (SD). Real time RT-PCR revealed minimal changes in PTN/MK gene expression in the spinal cord after CCI despite the strain considered, but marked changes were detected in DRG. A significant upregulation of PTN gene expression occurred in injured DRG of the F344 strain, the only strain that recovers from CCI-induced mechanical allodynia 28 days after surgery. In contrast, PTN was found to be downregulated in injured DRG of SD rats, the most sensitive strain in behavioural studies. These changes in PTN were not paralleled by concomitant modifications of MK gene expression. The results demonstrate previously unidentified differences between PTN and MK patterns of expression. Furthermore, the data suggest that upregulation of PTN, but not MK, could play an important role in the recovery from CCI.
ISSN:0897-7194
1029-2292
DOI:10.1080/08977190801987711