Effect of β-casein, κ-casein and β-lactoglobulin genotypes on concentration of milk protein variants

Individual mid-lactation milk samples were collected from 116 cows of the Swedish Red and Swedish Holstein breeds with known genotypes of β-casein, κ-casein and β-lactoglobulin. Detailed milk protein composition and allele-specific expression of β-casein, κ-casein and β-lactoglobulin proteins in mil...

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Bibliographic Details
Published in:Journal of animal breeding and genetics (1986) 2008-04, Vol.125 (2), p.119-129
Main Authors: Hallén, E, Wedholm, A, Andrén, A, Lundén, A
Format: Article
Language:English
Subjects:
Alleles
Animals
Breeding
Caseins - analysis
Caseins - genetics
Cattle - genetics
Cattle - metabolism
Chromatography, High Pressure Liquid
Female
Gene Frequency
Genetic polymorphism
Genetic Variation
Genotype
Heterozygote
Lactoglobulins - analysis
Lactoglobulins - genetics
Milk - chemistry
milk protein composition
milk protein expression
RP-HPLC
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Summary:Individual mid-lactation milk samples were collected from 116 cows of the Swedish Red and Swedish Holstein breeds with known genotypes of β-casein, κ-casein and β-lactoglobulin. Detailed milk protein composition and allele-specific expression of β-casein, κ-casein and β-lactoglobulin proteins in milk were analysed using reversed-phase high-performance liquid chromatography. Aggregate β-/κ-casein genotype was significantly associated with the κ-casein concentration in milk. The lowest κ-casein concentration was found in milk of cows with genotypes including κ-casein E (A¹A²/AE, A¹A¹/AE) and also A²A²/AA milk, whereas highest levels were associated with genotypes including κ-casein B. Casein number was positively and concentration of β-lactoglobulin negatively associated with the β-lactoglobulin BB genotype. In heterozygote cows, β-casein A¹ and β-lactoglobulin A proteins were found at higher concentrations in milk compared with the protein variant encoded by the alternative allele at these loci, whereas κ-casein A and B variants were found at similar concentrations in heterozygote AB cows.
ISSN:0931-2668
1439-0388
DOI:10.1111/j.1439-0388.2007.00706.x