Simple horizontal chamber for thermostated micro-thin-layer chromatography

The aim of presented work is to describe simple, fast and robust temperature-controlled system for non-forced-flow micro-planar chromatography. With this separation system the micro-TLC plates can be developed in horizontal position under temperature gradient or non-gradient as well as saturated or...

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Bibliographic Details
Published in:Journal of Chromatography A 2008-04, Vol.1187 (1), p.250-259
Main Author: Zarzycki, Paweł K.
Format: Article
Language:English
Subjects:
Analysis
Analytical chemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Thin Layer - instrumentation
Cyclodextrins
Estrogens
Exact sciences and technology
Fullerenes
Fullerenes - analysis
General pharmacology
Herbs extracts
Macrocycles
Medical sciences
Microchemistry - instrumentation
Other chromatographic methods
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Planar chromatography
Plant Extracts - analysis
Solid phase extraction
Steroids
Temperature
Testosterone
Thermostated chamber
Two-dimensional chromatography
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Summary:The aim of presented work is to describe simple, fast and robust temperature-controlled system for non-forced-flow micro-planar chromatography. With this separation system the micro-TLC plates can be developed in horizontal position under temperature gradient or non-gradient as well as saturated or unsaturated chamber conditions using low amount of mobile phase ranging from 0.3 to 1.0 mL. The device may work at wide range of temperatures from −20 to 80 °C. Under such conditions the plate temperature equilibration can be obtained within 5–12 min and a typical non-forced flow run can be finished within short period of time ranging from 5 to 20 min. It has been revealed that micro-plate is capable to separate more than 10 spots in one direction or up to 180 spots per plate for two-dimensional and multi-development runs. Particularly, fast and efficient separation of number of analytes including fullerenes, cyclodextrins and steroids as well as complex samples obtained from natural products and pharmaceutical formulations was demonstrated. Moreover, the application of thermostated micro-planar chromatography for the retention and quantification studies is also discussed.
ISSN:0021-9673
DOI:10.1016/j.chroma.2008.02.013