Effect of high levels of dietary vitamin C on the blood responses of matrinxã ( Brycon amazonicus)

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 2007-06, Vol.147 (2), p.383-388
• Main Authors: Affonso, Elizabeth Gusmão, Silva, Elisângela da Costa, Tavares-Dias, Marcos, de Menezes, Glauber Cruz, de Carvalho, Cristiane Suely Melo, Nunes, Érica da Silva Santiago, Ituassú, Daniel Rebelo, Roubach, Rodrigo, Ono, Eduardo Akifumi, Fim, Jorge Daniel Indrusiak, Marcon, Jaydione Luiz
• Format: Article
• Language: English
• Subjects: Animals; Ascorbic Acid - pharmacology; Ascorbic-acid; Blood; Blood Cells - cytology; Blood Cells - drug effects; Blood Glucose - analysis; Blood Proteins - analysis; Body Weight - drug effects; Brycon amazonicus; Cell Size - drug effects; Culture; Dietary Supplements; Erythrocyte Count; Fishes - blood; Freshwater; Hematocrit; Hemoglobins; Leukocyte Count; Leukocytes; Matrinxã
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/j.cbpa.2007.01.004

Juvenile matrinxã ( Brycon amazonicus) were fed with different dietary vitamin C (ascorbic acid or AA) concentrations for 2 months in order to evaluate its effect on the physiological condition of this species. Five treatments were tested: Reference diet–RD (350 mg AA kg − 1 and 45% crude protein commercial feed); C400, C500, C600 and C800 with supplementation of 400, 500, 600 and 800 mg AA kg − 1 . Fish fed 800 mg AA kg − 1 supplemented diets presented greater weight gain and survival. Hematocrit, hemoglobin concentrations and erythrocytes showed significant increase ( p < 0.05) in C600 and C800 relatively to the others. Total leukocyte values were significantly higher in fish fed vitamin C supplemented diets relatively to those fed RD and leukocytes count of C600 and C800 were the highest among the treatments. Monocytes were the only cells of fish defense mechanism that presented variation, and C600 and C800 presented the lowest values ( P < 0.05). Glucose level was significantly different relatively to RD only in C400 and the plasmatic total protein values for fish fed vitamin C supplemented diets were higher than for fish fed the RD. Fish plasmatic ions (Na + and Cl −) were significantly lower for C500, C600 and C800 relatively to C400 and RD. The physiological profile and the growth performance of matrinxã suggest that diet supplemented with 800 mg AA kg − 1 can improve its performance and increase the survival rate.