Cloning and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene and the use of its promoter for expression in Myrothecium gramineum, a novel expression host

At our laboratory, research has focused on the development of Myrothecium gramineum as a novel expression host. The glyceraldehyde-3-phosphate dehydrogenase (gpd)-promoter of M. gramineum was isolated and characterized (Genbank accession number EF486690). In order to prove its functionality and to e...

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Bibliographic Details
Published in:FEMS microbiology letters 2008-04, Vol.281 (2), p.140-146
Main Authors: De Maeseneire, Sofie L, Dauvrin, Thierry, Jonniaux, Jean-Luc, Van Bogaert, Inge N.A, Soetaert, Wim K, Vandamme, Erick J.M.C
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amylases
Amylases - genetics
Amylases - metabolism
Aspergillus oryzae - enzymology
Aspergillus oryzae Taka-amylase
Base Sequence
Biological and medical sciences
Cloning
Cloning, Molecular
Dehydrogenase
Dehydrogenases
DNA, Fungal - chemistry
DNA, Fungal - genetics
expression system
Fundamental and applied biological sciences. Psychology
Fungi
Gene Dosage
Gene Expression
Genetic Vectors - genetics
Glyceraldehyde-3-phosphate dehydrogenase
Glyceraldehyde-3-Phosphate Dehydrogenases - chemistry
Glyceraldehyde-3-Phosphate Dehydrogenases - genetics
Homology
Hypocreales - genetics
Microbiology
Molecular Sequence Data
Myrothecium
Myrothecium gramineum
Promoter Regions, Genetic
Sequence Analysis, DNA
Transformation, Genetic
α-Amylase
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Summary:At our laboratory, research has focused on the development of Myrothecium gramineum as a novel expression host. The glyceraldehyde-3-phosphate dehydrogenase (gpd)-promoter of M. gramineum was isolated and characterized (Genbank accession number EF486690). In order to prove its functionality and to explore the potential of M. gramineum as a novel fungal expression host, use of this gpd-promoter for the expression of a fungal α-amylase was investigated. Myrothecium gramineum was transformed with pGPDlpAmyAO, containing the gpd-promoter followed by the amy3 encoding sequence of Aspergillus oryzae. Study of the amylase production indicated that the promoter can be successfully used for the expression of heterologous proteins in M. gramineum. To the best of our knowledge, this is the first time a homologous expression system has been described for M. gramineum.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2008.01091.x