An anti-inflammatory effect of murine fetal liver cells in BALB/c mouse contact hypersensitivity model

• Published in: International immunopharmacology 2007-06, Vol.7 (6), p.744-749
• Main Authors: Biziulevičienė, Genė, Puidokaitė, Gintarė, Šiaurys, Almantas, Mauricas, Mykolas
• Format: Article
• Language: English
• Subjects: ADP-ribosyl Cyclase 1 - immunology; Animals; Anti-inflammatory action; Anti-Inflammatory Agents - pharmacology; Biological and medical sciences; Contact hypersensitivity reaction; Dermatitis, Contact - drug therapy; Dermatitis, Contact - pathology; Dinitrofluorobenzene; Disease Models, Animal; Female; Hematopoietic Stem Cells - immunology; Interleukin-10 - blood; Interleukin-10 - immunology; Liver - cytology; Liver - embryology; Medical sciences; Mice; Mice, Inbred BALB C; Murine fetal liver cells; Organ Size - drug effects; Paw edema; Pharmacology. Drug treatments; Proto-Oncogene Proteins c-kit - immunology; Spleen - drug effects; Spleen - growth & development; Thymus Gland - drug effects; Thymus Gland - growth & development; Tumor Necrosis Factor-alpha - blood; Tumor Necrosis Factor-alpha - immunology
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2007.01.012

Anti-inflammatory effects of murine fetal liver (FL) cells were studied using BALB/c mouse contact hypersensitivity (paw edema) model. Paw weight differences, lymphatic organ weights, hematological and histological indices as well as proinflammatory (TNF-α) and anti-inflammatory (IL-10) cytokine levels in sera were evaluated. Immunophenotyping revealed that both murine FL homogenate cells (HC) and FL hematopoietic stem cells (HSC) express CD117 and CD38 surface markers. Single doses of 1×106 cells/mouse and 2×106 cells/mouse of FL HC as well as of FL HSC, when used separately, all statistically significantly (p