Use of the gyrB gene to discriminate among species of the Burkholderia cepacia complex

Bacteria of the Burkholderia cepacia complex (Bcc) are opportunistic pathogens that can cause serious infections in lungs of cystic fibrosis patients. The Bcc comprises at least nine species that have been discriminated by a polyphasic taxonomic approach. In this study, we focused on the gyrB gene,...

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Bibliographic Details
Published in:FEMS microbiology letters 2008-04, Vol.281 (2), p.175-182
Main Authors: Tabacchioni, Silvia, Ferri, Lorenzo, Manno, Graziana, Mentasti, Massimo, Cocchi, Priscilla, Campana, Silvia, Ravenni, Novella, Taccetti, Giovanni, Dalmastri, Claudia, Chiarini, Luigi, Bevivino, Annamaria, Fani, Renato
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - genetics
Biological and medical sciences
Burkholderia cepacia
Burkholderia cepacia complex
Burkholderia cepacia complex - classification
Burkholderia cepacia complex - genetics
Burkholderia cepacia complex - isolation & purification
Burkholderia Infections - microbiology
Cystic fibrosis
Deoxyribonucleic acid
DNA
DNA Gyrase - genetics
DNA Primers
DNA topoisomerase
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
gyrB
Humans
identification
Microbiology
Molecular Sequence Data
Nucleotides
Opportunist infection
Phylogeny
Polymerase Chain Reaction
Polymorphism, Genetic
Sequence Analysis, DNA
Species
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Summary:Bacteria of the Burkholderia cepacia complex (Bcc) are opportunistic pathogens that can cause serious infections in lungs of cystic fibrosis patients. The Bcc comprises at least nine species that have been discriminated by a polyphasic taxonomic approach. In this study, we focused on the gyrB gene, universally distributed among bacteria, as a new target gene to discriminate among the Bcc species. New PCR primers were designed to amplify a gyrB DNA fragment of about 1900 bp from 76 strains representative of all Bcc species. Nucleotide sequences of PCR products were determined and showed more than 400 polymorphic sites with high sequence similarity values from most isolates of the same species. Phylogenetic tree analysis revealed that most of the 76 gyrB sequences grouped, forming clusters, each corresponding to a given Bcc species.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2008.01105.x