Profiling of differentially expressed genes in porcine epithelial cells derived from periodontal ligament and gingiva by DNA microarray

Abstract Objective It is unknown which genes are differentially expressed in cultured epithelial cells derived from the epithelial rests of Malassez (ERM) in periodontal ligament and oral gingival epithelium (OGE). This study analysed the different gene expression of OGE and ERM cells using a DNA mi...

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Published in:Archives of oral biology 2008-05, Vol.53 (5), p.437-442
Main Authors: Kurashige, Yoshihito, Saitoh, Masato, Nishimura, Michiko, Noro, Daisuke, Kaku, Tohru, Igarashi, Seiji, Takuma, Taishin, Arakawa, Toshiya, Inoue, Takashi, Abiko, Yoshihiro
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Cadherins - genetics
Cadherins - metabolism
Dentistry
Enzyme-Linked Immunosorbent Assay
Epithelial cell
Epithelial Cells - metabolism
Gene Expression Profiling
Gingiva
Gingiva - cytology
Gingiva - metabolism
Malassez
Microarray
Microarray Analysis
Periodontal ligament
Periodontal Ligament - cytology
Periodontal Ligament - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Swine - genetics
Thromboplastin - genetics
Thromboplastin - metabolism
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Summary:Abstract Objective It is unknown which genes are differentially expressed in cultured epithelial cells derived from the epithelial rests of Malassez (ERM) in periodontal ligament and oral gingival epithelium (OGE). This study analysed the different gene expression of OGE and ERM cells using a DNA microarray technique. Design Epithelial cells from ERM and OGE were isolated from porcine periodontal ligament and oral gingival epithelium. Each RNA sample extracted from the cells was reverse transcribed into cDNA and labelled with either cytidine 5-dUTP (Cy5) or cytidine 3-dUTP (Cy3). These labelled cDNA probes were then mixed and simultaneously hybridised to the Pig 13K microarray plate bearing 13,295 different genes (Operon, AL). Cellular enzyme-linked immunosorbent assay (CELISA) was performed to confirm the expression at protein level. Results There were nine genes common to the triplicate microarrays in ERM cells and one in OGE cells. Four of the nine genes including tissue factor (TF), FAT cadherin (FAT) and two unknown genes were expressed at levels more than threefold higher in ERM cells than in OGE cells. The protein levels of both TF and FAT in ERM cells were significantly higher than those in OGE. Conclusion TF and FAT may act as markers to distinguish ERM cells from OGE cells in vitro.
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2007.12.005