Rapid accumulation of nucleostemin in nucleolus during newt regeneration

In newt regeneration, differentiated cells can revert to stem cell–like cells in which the proliferative ability and multipotentiality are restored after dedifferentiation. However, the molecular events that occur during the dedifferentiation still remain obscure. Nucleostemin has been identified in...

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Bibliographic Details
Published in:Developmental dynamics 2007-04, Vol.236 (4), p.941-950
Main Authors: Maki, Nobuyasu, Takechi, Katsuaki, Sano, Shozo, Tarui, Hiroshi, Sasai, Yoshiki, Agata, Kiyokazu
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cell Differentiation - genetics
Cell Nucleus - metabolism
Cloning, Molecular
dedifferentiation
Embryo, Nonmammalian
Female
Gene Expression Regulation
Iris - physiology
Molecular Sequence Data
Muscle Fibers, Skeletal - cytology
newt
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
nucleolus
nucleostemin
Phylogeny
regeneration
Regeneration - genetics
Salamandridae - genetics
Salamandridae - metabolism
Salamandridae - physiology
Sequence Homology, Amino Acid
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Summary:In newt regeneration, differentiated cells can revert to stem cell–like cells in which the proliferative ability and multipotentiality are restored after dedifferentiation. However, the molecular events that occur during the dedifferentiation still remain obscure. Nucleostemin has been identified in mammals as a nucleolar protein specific to stem cells and cancer cells. In this study, a newt nucleostemin homologue was cloned and its regulation was analyzed. During lens regeneration, the expression of nucleostemin was activated and nucleostemin rapidly accumulated in the nucleoli of dedifferentiating pigmented epithelial cells 2 days before cell cycle reentry. During limb regeneration, nucleostemin also accumulated in the nucleoli of degenerating multinucleate muscle fibers before blastema formation. These findings suggest that nucleostemin plays a role in the dedifferentiation of newt cells and can provide crucial clues for addressing the molecular events at early steps of cellular dedifferentiation in newts. Developmental Dynamics 236:941–950, 2007. © 2006 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.21027