Molecular characterization of glutathione peroxidase gene from the liver of silver carp, bighead carp and grass carp

The cDNAs encoding glutathione peroxidase (GPx) were cloned and sequenced from the liver of three Chinese carps with different tolerance to hepatotoxic microcystins, phyto-planktivorous silver carp (Hypophtfialmichthys molitrix) and bighead carp (Aristichthys nobilis), and herbivorous grass carp (Ct...

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Bibliographic Details
Published in:BMB reports 2008-03, Vol.41 (3), p.204-209
Main Authors: Li, Guang-Zhao (Jinan University, Shipai, Guangzhou, China), Liang, Xu-Fang (Jinan University, Shipai, Guangzhou, China), E-mail: tliangxf@jnu.edu.cn, Yao, Wei (Jinan University, Shipai, Guangzhou, China), Liao, Wan-Qin (Jinan University, Shipai, Guangzhou, China), Zhu, Wei-Feng (Jinan University, Shipai, Guangzhou, China)
Format: Article
Language:English
Subjects:
5' Flanking Region - genetics
Amino Acid Sequence
Animals
Base Sequence
Carps - classification
Carps - genetics
China
Cloning, Molecular
FRESHWATER FISHES
Gene Expression Profiling
Gene Expression Regulation, Enzymologic
Glutathione Peroxidase - chemistry
Glutathione Peroxidase - genetics
Glutathione Peroxidase - metabolism
glutathione peroxidases
HYPOPHTHALMICHTHYS MOLITRIX
Liver - enzymology
liver expression
microcystin detoxification
Molecular Sequence Data
PECES DE AGUA DULCE
Phylogeny
POISSON D'EAU DOUCE
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Analysis, DNA
Sequence Homology, Amino Acid
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Summary:The cDNAs encoding glutathione peroxidase (GPx) were cloned and sequenced from the liver of three Chinese carps with different tolerance to hepatotoxic microcystins, phyto-planktivorous silver carp (Hypophtfialmichthys molitrix) and bighead carp (Aristichthys nobilis), and herbivorous grass carp (Ctenopharyngodon idellus). Using genome walker method, a 750 bp 5'-flanking region of the silver carp GPx gene was obtained, and several potential regulatory elements were identified in the promoter region of the GPx gene. The silver carp GPx gene was widely expressed in all tissues examined. Despite phylogenetic analysis, assigning this newly described carp GPx to the group of mammalian GPx2, the carp GPx seems more similar to GPx1 from a physiological point of view. The constitutive expression pattern of the three carp liver GPx gene, shows a positive relationship with their tolerance to microcystins.
ISSN:1976-6696
DOI:10.5483/BMBRep.2008.41.3.204