The effects of BmNPV on biochemical changes in primary cultures of Bombyx mori embryonic tissue

The effect of Bombyx mori nuclear polyhedrosis virus (BmNPV) on biochemical changes of TC-100 medium containing 10% fetal bovine serum (FBS) in embryonic primary cultures of silkworm was investigated. The primary cultures that reached 60% confluence were infected by 0.5, 1, and 2-ml viral inoculums...

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Published in:In vitro cellular & developmental biology. Animal 2008-03, Vol.44 (3), p.121-127
Main Authors: Matindoost, Leila, Sendi, Jalal Jalali, Soleimanjahi, Hoorieh, Etebari, Kayvan, Rahbarizade, Fateme
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Biochemical changes introduction
Biochemistry
Biomedical and Life Sciences
BmNPV
Bombyx - cytology
Bombyx - virology
Bombyx mori
Bombyx mori NPV
Cell Biology
Cell Culture
Cell culture techniques
Cell lines
Cells, Cultured
Cellular metabolism
Cellular Pathology/Virology
Cholesterol - metabolism
Culture Media
Cultured cells
Developmental Biology
Embryo, Nonmammalian - cytology
Embryo, Nonmammalian - virology
Glucose - metabolism
Infections
Insect cell
Insect pests
Insect viruses
Life Sciences
Nucleopolyhedrovirus - physiology
Primary culture
Stem Cells
Uric acid
Virus Replication
Viruses
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Summary:The effect of Bombyx mori nuclear polyhedrosis virus (BmNPV) on biochemical changes of TC-100 medium containing 10% fetal bovine serum (FBS) in embryonic primary cultures of silkworm was investigated. The primary cultures that reached 60% confluence were infected by 0.5, 1, and 2-ml viral inoculums (diluted with TC-100 medium representing multiplicity of infection (MOI) of 0.25, 0.5, and 1). Glucose, uric acid, urea, total protein, cholesterol, and alkaline phosphatase were measured in the medium of BmNPV-infected primary cultures. All biochemical compounds showed significant changes. Glucose decreased considerably by about 55 mg/ml, while different concentrations of the virus inoculums did not demonstrate significant differences among them. Total protein had only increased in 2 ml concentration and there were no changes in other concentrations. Uric acid as a by-product accumulated dramatically in all concentrations, while the amount of urea reduced in all treatments and this reduction was more evident in lower concentrations. Cholesterol consumption was high in cultures postinfection, while alkaline phospha-tase (ALP) activity decreased in infected cells.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-008-9083-3