VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration

Membrane trafficking is one of the most important mechanisms involved in the establishment and maintenance of the forms and functions of the cell. However, it is poorly understood in skeletal muscle cells. In this study, we have focused on vesicle-associated membrane proteins (VAMPs), which are comp...

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Bibliographic Details
Published in:Cell and tissue research 2007-06, Vol.328 (3), p.573-581
Main Authors: Tajika, Yuki, Sato, Mahito, Murakami, Tohru, Takata, Kuniaki, Yorifuji, Hiroshi
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Cellular biology
Gene expression
Male
Muscle Fibers, Skeletal - metabolism
Muscle, Skeletal - physiology
Muscular system
Rats
Rats, Wistar
Regeneration - genetics
Rodents
Satellite Cells, Skeletal Muscle - metabolism
Up-Regulation
Vesicle-Associated Membrane Protein 1 - metabolism
Vesicle-Associated Membrane Protein 2 - genetics
Vesicle-Associated Membrane Protein 2 - metabolism
Vesicle-Associated Membrane Protein 3 - metabolism
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Summary:Membrane trafficking is one of the most important mechanisms involved in the establishment and maintenance of the forms and functions of the cell. However, it is poorly understood in skeletal muscle cells. In this study, we have focused on vesicle-associated membrane proteins (VAMPs), which are components of the vesicle docking and fusion complex, and have performed immunostaining to investigate the expression of VAMPs in rat skeletal muscle tissue. We have found that VAMP2, but not VAMP1 or VAMP3, is expressed in satellite cells. VAMP2 is also expressed in myofibers in the soleus muscle and nerve endings. This is consistent with previous studies in which VAMP2 has been shown to regulate GLUT4 trafficking in slow-twitch myofibers in soleus muscle and neurotransmitter release in nerve endings. As satellite cells are quiescent myogenic cells, the expression of VAMP2 has further been examined in regenerating muscles after injury by the snake venom, cardiotoxin; we have observed enhanced expression of VAMP2 in immature myotubes with a peak at 3 days after injury. Our findings suggest that VAMP2 plays roles in quiescent satellite cells and is involved in muscle regeneration. The nature of the material transported in the VAMP2-bearing vesicles in satellite cells and myotubes is still under investigation.
ISSN:0302-766X
1432-0878
DOI:10.1007/s00441-006-0376-0