Long-Term Survival of HLA-DR15+ Pig Skin in SCID Mice After Reconstitution With Human Peripheral Blood Mononuclear Cells and Under Short-Term Immunosuppression

Abstract In allotransplantation, donor–recipient human leukocyte antigen (HLA) matches improve graft survival. For studies of the role of donor–recipient HLA II matching on xenotransplantation, we successfully generated HLA-DR15+ transgenic pigs the the skins of which were transplanted to SCID mice,...

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Bibliographic Details
Published in:Transplantation proceedings 2008-03, Vol.40 (2), p.570-573
Main Authors: Tai, H.-C, Tu, C.-F, Lee, J.-M, Ho, L.-L, Tseng, Y.-L, Chou, N.-K, Yang, T.-S, Weng, C.-N, Lee, P.-H, Chang, K.-J, Tang, Y.-B
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Graft Survival - immunology
HLA-DR Antigens - immunology
HLA-DR Serological Subtypes
Humans
Immunosuppression
Leukocytes, Mononuclear - immunology
Medical sciences
Mice
Mice, SCID
Skin - immunology
Skin Transplantation - immunology
Surgery
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Swine
Tissue, organ and graft immunology
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Summary:Abstract In allotransplantation, donor–recipient human leukocyte antigen (HLA) matches improve graft survival. For studies of the role of donor–recipient HLA II matching on xenotransplantation, we successfully generated HLA-DR15+ transgenic pigs the the skins of which were transplanted to SCID mice, which were thereafter reconstituted with HLA-DR15+ or -DR15− hPBMC. Cyclosporine was given intraperitoneally to SCID mice for 12 days. Human T cells were observed in SCID mice after reconstitution. Mixed lymphocytes responses showed greater responses by HLA-DR15− human peripheral blood mononuclear cells (hPBMC) against HLA-DR15+ porcine PBMC. HLA-DR15+ porcine skins survived more than 100 days in all SCID mice. HLA-DR15+ porcine skins were rejected in all non-SCID (Balb/c) mice. The histologic pictures of transplanted HLA-DR15+ porcine skins showed surviving porcine epithelium in remodeling murine dermis and little lymphocyte infiltration into the murine dermis. The long-term survival of HLA-DR15+ pig skin in all hPBMC-SCID mice might be due to poor engraftment or function of reconstituted T cells. Further studies are needed to clarify the role of donor-recipient matching of HLA-DR15.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2008.02.006