Up-regulation of Connexin43 in the glial scar following photothrombotic ischemic injury

Several types of CNS injury and various diseases are associated with the development of a glial scar. Astrocytes are major components of the glial scar. They are interconnected by gap junctions, with connexin43 (Cx43) being the most prominent channel protein. We applied a model of focal cerebral isc...

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Bibliographic Details
Published in:Molecular and cellular neuroscience 2007-05, Vol.35 (1), p.89-99
Main Authors: Haupt, Corinna, Witte, Otto W., Frahm, Christiane
Format: Article
Language:English
Subjects:
Animals
Astrocytes - metabolism
Astrocytes - pathology
Astrogliosis
Brain Ischemia - metabolism
Brain Ischemia - pathology
BrdU
Cell Division - physiology
Connexin 43 - genetics
Connexin 43 - metabolism
Gap junctions
Gap Junctions - metabolism
Gap Junctions - pathology
GFAP
Glial Fibrillary Acidic Protein - genetics
Glial Fibrillary Acidic Protein - metabolism
Gliosis - metabolism
Gliosis - pathology
Immunohistochemistry
In Situ Hybridization
Intracranial Thrombosis - metabolism
Intracranial Thrombosis - pathology
Male
Nestin
Rats
Rats, Wistar
Reactive astrocytes
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Up-Regulation - physiology
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Summary:Several types of CNS injury and various diseases are associated with the development of a glial scar. Astrocytes are major components of the glial scar. They are interconnected by gap junctions, with connexin43 (Cx43) being the most prominent channel protein. We applied a model of focal cerebral ischemia to study the spatio-temporal expression of glial fibrillary acidic protein, as well as of Cx43 mRNA and protein in gliotic tissue up to 60 days after injury. Reactive astrocytes enveloping the lesion up-regulated their Cx43 mRNA and protein. A band of reactive astrocytes filling in the lesion exhibited elevated Cx43 and showed a high degree of proliferation. Because of these findings, we hypothesize a role for Cx43 in glial scar formation, specifically in the proliferation of astrocytes.
ISSN:1044-7431
1095-9327
DOI:10.1016/j.mcn.2007.02.005