Flow injection determination of anisidine value in palm oil samples using a triiodide potentiometric detector

• Published in: Analytica chimica acta 2007-05, Vol.591 (2), p.248-254
• Main Authors: Saad, Bahruddin, Wai, Wan Tatt, Lim, Boey Peng, Saleh, Muhammad Idiris
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Anisidine value; Chemistry; Chloramine-T; Electrochemical methods; Exact sciences and technology; Flow injection analysis; General, instrumentation; Oxidative stability; Palm olein; Triiodide electrode
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2007.03.067

A flow injection analysis (FIA) procedure for the determination of anisidine value (AV) in palm olein using a triiodide detector is described. Undiluted oil sample and chloramine-T reagent were added to a reaction chamber, and reaction was accelerated by applying a short vortex action (typically for 30 s). After allowing the emulsified oil phase to be separated from the aqueous phase (bottom layer), an aliquot of the aqueous phase (containing unreacted chloramine-T) was aspirated into a carrier stream that contained I − where the chloramine-T oxidized the I − to form I 3 − which was finally detected by a flow-through triiodide potentiometric detector. Variables that affect the FIA signals such as size of the reaction chamber, oil and reagent flow rates, chloramine-T concentration, vortex time, time for phase separation, carrier stream pH and injected volume were studied. The optimized FIA procedure is linear over 1.0–23.0 AV. The method exhibits good repeatabililty (R.S.D. of ±3.16% ( n = 4) for the determination of 5.0 AV) and a sampling rate of 40 samples per hour was achieved. Good correlation ( r 2 = 0.996 ( n = 4)) between the proposed method and the manual American Oil Chemists’ Society procedure was found when applied to the determination of twenty different types of palm olein samples.