Triphlorethol-A induces heme oxygenase-1 via activation of ERK and NF-E2 related factor 2 transcription factor

• Published in: FEBS letters 2007-05, Vol.581 (10), p.2000-2008
• Main Authors: Kang, Kyoung Ah, Lee, Kyoung Hwa, Park, Jae Woo, Lee, Nam Ho, Na, Hye Kyung, Surh, Young Joon, You, Ho Jin, Chung, Myung Hee, Hyun, Jin Won
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant response element; Cell Death - drug effects; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Cells, Cultured; CHO Cells; Cricetinae; Cricetulus; Ecklonia cava; Enzyme Activation - drug effects; Enzyme Induction - drug effects; Extracellular Signal-Regulated MAP Kinases - metabolism; Heme oxygenase-1; Heme Oxygenase-1 - biosynthesis; Heme Oxygenase-1 - genetics; Models, Biological; NF-E2 related factor 2; NF-E2-Related Factor 2 - metabolism; Oxidative Stress - drug effects; Phloroglucinol - analogs & derivatives; Phloroglucinol - chemistry; Phloroglucinol - pharmacology; Phosphorylation - drug effects; Protein Transport - drug effects; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transcription, Genetic - drug effects; Triphlorethol-A
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2007.04.022

Triphlorethol-A, phlorotannin found in Ecklonia cava, induced heme oxygenase-1 (HO-1) expression at mRNA and protein levels, leading to increased HO-1 activity. Transcription factor NF-E2 related factor 2 (Nrf2) regulates antioxidant response element (ARE) of phase 2 detoxifying and antioxidant enzymes. Triphlorethol-A increased nuclear translocation, ARE binding, and transcriptional activity of Nrf2. Triphlorethol-A exhibited activation of ERK and U0126, inhibitor of ERK kinase, suppressed triphlorethol-A induced activation of Nrf2, finally decreased HO-1 protein level. Taken together, these data suggest that triphlorethol-A augments cellular antioxidant defense capacity through induction of HO-1 via ERK-Nrf2-ARE signaling pathway, thereby protecting cells from oxidative stress.