Formulation and cytotoxicity of doxorubicin loaded in self-assembled bio-polyelectrolyte microshells

A bio-polyelectrolyte microshell composed of alginate sodium (ALG) and chitosan (CHI) was fabricated by electrostatic layer-by-layer (LbL) self-assembly technique. The resulting ALG-CHI microshells were found to be able to effectively load anti-cancer drug doxorubicin (DOX) in the interior of the sh...

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Bibliographic Details
Published in:International journal of pharmaceutics 2007-05, Vol.336 (2), p.376-381
Main Authors: Tao, Xia, Chen, Hua, Sun, Xue-Jun, Chen, Jian-Feng, Roa, Wilson H.
Format: Article
Language:English
Subjects:
Alginates - chemistry
Antibiotics, Antineoplastic - administration & dosage
Antibiotics, Antineoplastic - chemistry
Antibiotics, Antineoplastic - pharmacology
Bio-polyelectrolyte
Biological and medical sciences
Cell Line, Tumor
Cell Survival - drug effects
Chitosan - chemistry
Colorimetry
Cytotoxicity
Delayed-Action Preparations
Doxorubicin
Doxorubicin - administration & dosage
Doxorubicin - chemistry
Doxorubicin - pharmacology
Drug Carriers - chemistry
Electrolytes
General pharmacology
Glucuronic Acid - chemistry
Hexuronic Acids - chemistry
Humans
Medical sciences
Microscopy, Confocal
Microshell
Microspheres
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Self-assembly
Static Electricity
Technology, Pharmaceutical
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Description
Summary:A bio-polyelectrolyte microshell composed of alginate sodium (ALG) and chitosan (CHI) was fabricated by electrostatic layer-by-layer (LbL) self-assembly technique. The resulting ALG-CHI microshells were found to be able to effectively load anti-cancer drug doxorubicin (DOX) in the interior of the shells under modest conditions without addition of other reagents, as demonstrated by confocal laser scanning microscopy (CLSM). The mass of DOX loaded in one capsule of four alginate/chitosan layers (i.e. the volume V = 2.5 × 10 −10 cm 3) is calculated as ca. 1.4 × 10 −13 g, which corresponds to 1.5 × 10 8 DOX molecules. Also, the release of DOX in the shells is dependent on the number of assembled layers of the shells. Colorimetric XTT cell viability assay results showed that the DOX-loaded microshells at high concentrations tested could kill cancer cells more efficiently than free-DOX alone.
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2006.12.009