Nuclear Progesterone Receptors in the Human Pregnancy Myometrium: Evidence that Parturition Involves Functional Progesterone Withdrawal Mediated by Increased Expression of Progesterone Receptor-A

Context: We examined whether human parturition involves functional progesterone withdrawal mediated by changes in myometrial expression of progesterone receptors (PRs)-A and -B. Objective: Our objectives were to: 1) measure PR-A and PR-B protein levels in human pregnancy myometrium and determine whe...

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Published in:The journal of clinical endocrinology and metabolism 2007-05, Vol.92 (5), p.1927-1933
Main Authors: Merlino, Amy A., Welsh, Toni N., Tan, Huiqing, Yi, Li Juan, Cannon, Vernon, Mercer, Brian M., Mesiano, Sam
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Blotting, Western
Cell Line
Cell Nucleus - metabolism
Endocrinopathies
Female
Fundamental and applied biological sciences. Psychology
Humans
Immunohistochemistry
Medical sciences
Myometrium - cytology
Myometrium - metabolism
Parturition - physiology
Plasmids - genetics
Pregnancy - metabolism
Progesterone - physiology
Receptors, Cytoplasmic and Nuclear - metabolism
Receptors, Progesterone - biosynthesis
RNA, Small Interfering - genetics
Transfection
Vertebrates: endocrinology
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Summary:Context: We examined whether human parturition involves functional progesterone withdrawal mediated by changes in myometrial expression of progesterone receptors (PRs)-A and -B. Objective: Our objectives were to: 1) measure PR-A and PR-B protein levels in human pregnancy myometrium and determine whether the PR-A to PR-B ratio changes with advancing gestation and labor onset; and 2) determine how changes in the PR-A to PR-B ratio affect myometrial cell progesterone responsiveness. Design: PR protein levels and cellular localization were measured by Western blotting and immunohistochemistry, respectively, in lower uterine segment uterine wall tissue from preterm (
ISSN:0021-972X
1945-7197
DOI:10.1210/jc.2007-0077