Enhanced micropropagation response and biocontrol effect of Azospirillum brasilense Sp245 on Prunus cerasifera L. clone Mr.S 2/5 plants

• Published in: Journal of biotechnology 2008-04, Vol.134 (3), p.312-319
• Main Authors: Russo, Anna, Vettori, Lorenzo, Felici, Cristiana, Fiaschi, Grazia, Morini, Stefano, Toffanin, Annita
• Format: Article
• Language: English
• Subjects: Acclimatization - physiology; Antibiosis - physiology; Azospirillum brasilense; Azospirillum brasilense - physiology; BCA; Biological and medical sciences; biological control; Biomass; Biotechnology; Clone Cells; disease resistance; explants; Fundamental and applied biological sciences. Psychology; fungal antagonists; fungal diseases of plants; Genes, Fungal; IAA; Incubators; Indoles - pharmacology; inoculation methods; Micropropagation; Pest Control, Biological - methods; PGPR; Plant Diseases - microbiology; Plant Diseases - parasitology; plant growth-promoting rhizobacteria; Plant Roots - growth & development; Plant Roots - microbiology; Plant Roots - parasitology; Plant Shoots - growth & development; Plant Shoots - microbiology; Plant Shoots - parasitology; Prunus; Prunus - growth & development; Prunus - microbiology; Prunus - parasitology; Prunus cerasifera; Rhizoctonia; Rhizoctonia - cytology; Rhizoctonia - pathogenicity; root growth; root hairs; rooting; tissue culture
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.jbiotec.2008.01.020

Inoculation with Azospirillum brasilense Sp245 exerts beneficial effects on micropropagated plants of Prunus cerasifera L. clone Mr.S 2/5, as seen in the results of a comparative analysis of inoculated and non-inoculated explants, during both the rooting and acclimatation phases. The presence of Azospirillum brasilense Sp245 increased root system, root hair biomass production and apical activity. Although the presence of the bacteria had a positive effect on rooting, the addition of indolebutyric acid (IBA) to Murashige and Skoog (MS) medium was seen as indispensable in order to promote the rooting of explants. Aside from the promotion of plant growth, A. brasilense Sp245 protects plants against pathogen attacks, such as Rhizoctonia spp., with a plant survival rate of nearly 100% vs. 0% as seen in the negative control. The biocontrol effect of A. brasilense Sp245 on the fungal rhizospheric community has been confirmed by denaturing gradient gel electrophoresis (DGGE) profiles of the rhizospheric microbial community. This study indicates that A. brasilense Sp245 could be employed as a tool in plant biotechnology.