Increased expression of highly sulfated keratan sulfate synthesized in malignant astrocytic tumors

Keratan sulfate (KS) proteoglycans are expressed on a subpopulation of microglia in normal adult brain. We previously showed the up-regulated expression of KS in one of glioblastoma cell lines using anti-KS antibody (5D4). However, it has not been clarified whether KS is expressed in brain tumors an...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-05, Vol.369 (4), p.1041-1046
Main Authors: Kato, Yukinari, Hayatsu, Norihito, Kaneko, Mika Kato, Ogasawara, Satoshi, Hamano, Tetsutaro, Takahashi, Satoru, Nishikawa, Ryo, Matsutani, Masao, Mishima, Kazuhiko, Narimatsu, Hisashi
Format: Article
Language:English
Subjects:
Astrocytic tumor
Astrocytoma - diagnosis
Astrocytoma - metabolism
Astrocytoma - pathology
Cell Membrane - chemistry
Cell Membrane - metabolism
Central Nervous System Neoplasms - diagnosis
Central Nervous System Neoplasms - metabolism
Central Nervous System Neoplasms - pathology
Gene Expression
Glioblastoma
Glycogene
Humans
Immunohistochemistry
Keratan sulfate
Keratan Sulfate - analysis
Keratan Sulfate - genetics
Keratan Sulfate - metabolism
Prognosis
Proteoglycan
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Summary:Keratan sulfate (KS) proteoglycans are expressed on a subpopulation of microglia in normal adult brain. We previously showed the up-regulated expression of KS in one of glioblastoma cell lines using anti-KS antibody (5D4). However, it has not been clarified whether KS is expressed in brain tumors and is involved in their malignancy. In this study, 54 astrocytic tumors were investigated about KS-expression using Western-blot with 5D4. In six of 14 anaplastic astrocytomas (43%) and 23 of 34 glioblastomas (68%), KS was detected by 5D4. KS was hardly detected by 5D4 in diffuse astrocytoma, suggesting that KS-expression is significantly expressed in malignant astrocytic tumors. In immunohistochemistry, KS is highly expressed in cell surface of malignant astrocytic tumors. Taken together, KS might be associated with the malignancy of astrocytic tumors, and be useful for a prognostic factor of astrocytic tumors.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.02.130