Increased expression of highly sulfated keratan sulfate synthesized in malignant astrocytic tumors

• Published in: Biochemical and biophysical research communications 2008-05, Vol.369 (4), p.1041-1046
• Main Authors: Kato, Yukinari, Hayatsu, Norihito, Kaneko, Mika Kato, Ogasawara, Satoshi, Hamano, Tetsutaro, Takahashi, Satoru, Nishikawa, Ryo, Matsutani, Masao, Mishima, Kazuhiko, Narimatsu, Hisashi
• Format: Article
• Language: English
• Subjects: Astrocytic tumor; Astrocytoma - diagnosis; Astrocytoma - metabolism; Astrocytoma - pathology; Cell Membrane - chemistry; Cell Membrane - metabolism; Central Nervous System Neoplasms - diagnosis; Central Nervous System Neoplasms - metabolism; Central Nervous System Neoplasms - pathology; Gene Expression; Glioblastoma; Glycogene; Humans; Immunohistochemistry; Keratan sulfate; Keratan Sulfate - analysis; Keratan Sulfate - genetics; Keratan Sulfate - metabolism; Prognosis; Proteoglycan
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2008.02.130

Keratan sulfate (KS) proteoglycans are expressed on a subpopulation of microglia in normal adult brain. We previously showed the up-regulated expression of KS in one of glioblastoma cell lines using anti-KS antibody (5D4). However, it has not been clarified whether KS is expressed in brain tumors and is involved in their malignancy. In this study, 54 astrocytic tumors were investigated about KS-expression using Western-blot with 5D4. In six of 14 anaplastic astrocytomas (43%) and 23 of 34 glioblastomas (68%), KS was detected by 5D4. KS was hardly detected by 5D4 in diffuse astrocytoma, suggesting that KS-expression is significantly expressed in malignant astrocytic tumors. In immunohistochemistry, KS is highly expressed in cell surface of malignant astrocytic tumors. Taken together, KS might be associated with the malignancy of astrocytic tumors, and be useful for a prognostic factor of astrocytic tumors.