Easily fabricated magnetic traps for single-cell applications

We describe a simple and inexpensive method of fabricating single cell magnetic traps within a polydimethylsiloxane (PDMS) device. These traps were developed as part of an automated system that captures individual yeast cells in a microfluidic device and analyzes each cell as it buds. To make the tr...

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Bibliographic Details
Published in:Review of scientific instruments 2007-04, Vol.78 (4), p.044301-044301-5
Main Authors: Koschwanez, John H., Carlson, Robert H., Meldrum, Deirdre R.
Format: Article
Language:English
Subjects:
Alloys - chemistry
Catalysis
Cells, Immobilized - ultrastructure
Dimethylpolysiloxanes - chemistry
Magnetics
Microfluidic Analytical Techniques
Microscopy, Atomic Force
Palladium - chemistry
Saccharomycetales - growth & development
Saccharomycetales - ultrastructure
Silicones - chemistry
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Summary:We describe a simple and inexpensive method of fabricating single cell magnetic traps within a polydimethylsiloxane (PDMS) device. These traps were developed as part of an automated system that captures individual yeast cells in a microfluidic device and analyzes each cell as it buds. To make the traps, PdCl 2 catalyst is rubbed with vinyl foam onto plasma-patterned PDMS, and then Co-Ni-B alloy is electrolessly deposited onto the catalyst at a moderate temperature. We demonstrate individual yeast cell capture and estimate the capture force ( 1.9 − 4.4 pN ) by measuring the flow speed required to remove the cell from its trap in a microfluidic channel.
ISSN:0034-6748
1089-7623
DOI:10.1063/1.2722400