Wash-free, antibody-assisted magnetoreduction assays of orchid viruses

This study demonstrates the feasibility of wash-free magnetoreduction assays (MRA) of orchid viruses. A magnetic reagent, consisting of magnetic beads coated with antibodies and dispersed in water, was synthesized. By using a mixed-frequency alternative-current (ac) magnetosusceptometer, differences...

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Bibliographic Details
Published in:Journal of virological methods 2008-05, Vol.149 (2), p.334-337
Main Authors: Yang, S.Y., Jian, Z.F., Chieh, J.J., Horng, H.E., Yang, H.C., Huang, I.J., Hong, Chin-Yih
Format: Article
Language:English
Subjects:
Antibodies, Viral - metabolism
Assay
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Immunoassay - methods
Magnetic beads
Magnetics
Magnetoreduction
Microbiology
Microspheres
Orchid virus
Orchidaceae
Orchidaceae - virology
Plant Viruses - isolation & purification
Techniques used in virology
Virology
Virology - methods
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Summary:This study demonstrates the feasibility of wash-free magnetoreduction assays (MRA) of orchid viruses. A magnetic reagent, consisting of magnetic beads coated with antibodies and dispersed in water, was synthesized. By using a mixed-frequency alternative-current (ac) magnetosusceptometer, differences in the magnetic susceptibilities of the magnetic reagent before and after the addition of orchid solutions were measured. The results show significant advantages for MRA of orchid viruses.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2008.01.019