Loading…
Wash-free, antibody-assisted magnetoreduction assays of orchid viruses
This study demonstrates the feasibility of wash-free magnetoreduction assays (MRA) of orchid viruses. A magnetic reagent, consisting of magnetic beads coated with antibodies and dispersed in water, was synthesized. By using a mixed-frequency alternative-current (ac) magnetosusceptometer, differences...
Saved in:
| Published in: | Journal of virological methods 2008-05, Vol.149 (2), p.334-337 |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c475t-36d2381f710851b9d0d55d242e5fa343461b7f540fcc72c651c0e5a94d3d41e33 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c475t-36d2381f710851b9d0d55d242e5fa343461b7f540fcc72c651c0e5a94d3d41e33 |
| container_end_page | 337 |
| container_issue | 2 |
| container_start_page | 334 |
| container_title | Journal of virological methods |
| container_volume | 149 |
| creator | Yang, S.Y. Jian, Z.F. Chieh, J.J. Horng, H.E. Yang, H.C. Huang, I.J. Hong, Chin-Yih |
| description | This study demonstrates the feasibility of wash-free magnetoreduction assays (MRA) of orchid viruses. A magnetic reagent, consisting of magnetic beads coated with antibodies and dispersed in water, was synthesized. By using a mixed-frequency alternative-current (ac) magnetosusceptometer, differences in the magnetic susceptibilities of the magnetic reagent before and after the addition of orchid solutions were measured. The results show significant advantages for MRA of orchid viruses. |
| doi_str_mv | 10.1016/j.jviromet.2008.01.019 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70474643</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0166093408000281</els_id><sourcerecordid>20777558</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-36d2381f710851b9d0d55d242e5fa343461b7f540fcc72c651c0e5a94d3d41e33</originalsourceid><addsrcrecordid>eNqFkEtrFEEQgJugJGvMXwhz0ZOzVr9nbkpITCDgRfHY9HbXJL3sTMeumcD-ezvsqsdAQRXUVw8-xi45rDlw83m73j6nkkec1wKgWwOv0Z-wFe9s30LfqTdsVUFTa6nO2DuiLQBoK-UpO-OdNFZovWI3vzw9tkNB_NT4aU6bHPetJ0o0Y2xG_zDhnAvGJcwpT03t-D01eWhyCY8pNvWJhZDes7eD3xFeHPM5-3lz_ePqtr3__u3u6ut9G5TVcytNFLLjg-XQab7pI0Sto1AC9eClksrwjR20giEEK4LRPABq36soo-Io5Tn7eNj7VPLvBWl2Y6KAu52fMC_kLCirjHodFGCt1bqroDmAoWSigoN7Kmn0Ze84uBfVbuv-qnYvqh3wGn0dvDxeWDYjxv9jR7cV-HAEPAW_G4qfQqJ_nABhpAVTuS8HDqu454TFUUg4BYypYJhdzOm1X_4A9h2gAQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20777558</pqid></control><display><type>article</type><title>Wash-free, antibody-assisted magnetoreduction assays of orchid viruses</title><source>ScienceDirect Journals</source><creator>Yang, S.Y. ; Jian, Z.F. ; Chieh, J.J. ; Horng, H.E. ; Yang, H.C. ; Huang, I.J. ; Hong, Chin-Yih</creator><creatorcontrib>Yang, S.Y. ; Jian, Z.F. ; Chieh, J.J. ; Horng, H.E. ; Yang, H.C. ; Huang, I.J. ; Hong, Chin-Yih</creatorcontrib><description>This study demonstrates the feasibility of wash-free magnetoreduction assays (MRA) of orchid viruses. A magnetic reagent, consisting of magnetic beads coated with antibodies and dispersed in water, was synthesized. By using a mixed-frequency alternative-current (ac) magnetosusceptometer, differences in the magnetic susceptibilities of the magnetic reagent before and after the addition of orchid solutions were measured. The results show significant advantages for MRA of orchid viruses.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/j.jviromet.2008.01.019</identifier><identifier>PMID: 18367255</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Antibodies, Viral - metabolism ; Assay ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Immunoassay - methods ; Magnetic beads ; Magnetics ; Magnetoreduction ; Microbiology ; Microspheres ; Orchid virus ; Orchidaceae ; Orchidaceae - virology ; Plant Viruses - isolation & purification ; Techniques used in virology ; Virology ; Virology - methods</subject><ispartof>Journal of virological methods, 2008-05, Vol.149 (2), p.334-337</ispartof><rights>2008 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-36d2381f710851b9d0d55d242e5fa343461b7f540fcc72c651c0e5a94d3d41e33</citedby><cites>FETCH-LOGICAL-c475t-36d2381f710851b9d0d55d242e5fa343461b7f540fcc72c651c0e5a94d3d41e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20263706$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18367255$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, S.Y.</creatorcontrib><creatorcontrib>Jian, Z.F.</creatorcontrib><creatorcontrib>Chieh, J.J.</creatorcontrib><creatorcontrib>Horng, H.E.</creatorcontrib><creatorcontrib>Yang, H.C.</creatorcontrib><creatorcontrib>Huang, I.J.</creatorcontrib><creatorcontrib>Hong, Chin-Yih</creatorcontrib><title>Wash-free, antibody-assisted magnetoreduction assays of orchid viruses</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>This study demonstrates the feasibility of wash-free magnetoreduction assays (MRA) of orchid viruses. A magnetic reagent, consisting of magnetic beads coated with antibodies and dispersed in water, was synthesized. By using a mixed-frequency alternative-current (ac) magnetosusceptometer, differences in the magnetic susceptibilities of the magnetic reagent before and after the addition of orchid solutions were measured. The results show significant advantages for MRA of orchid viruses.</description><subject>Antibodies, Viral - metabolism</subject><subject>Assay</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunoassay - methods</subject><subject>Magnetic beads</subject><subject>Magnetics</subject><subject>Magnetoreduction</subject><subject>Microbiology</subject><subject>Microspheres</subject><subject>Orchid virus</subject><subject>Orchidaceae</subject><subject>Orchidaceae - virology</subject><subject>Plant Viruses - isolation & purification</subject><subject>Techniques used in virology</subject><subject>Virology</subject><subject>Virology - methods</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkEtrFEEQgJugJGvMXwhz0ZOzVr9nbkpITCDgRfHY9HbXJL3sTMeumcD-ezvsqsdAQRXUVw8-xi45rDlw83m73j6nkkec1wKgWwOv0Z-wFe9s30LfqTdsVUFTa6nO2DuiLQBoK-UpO-OdNFZovWI3vzw9tkNB_NT4aU6bHPetJ0o0Y2xG_zDhnAvGJcwpT03t-D01eWhyCY8pNvWJhZDes7eD3xFeHPM5-3lz_ePqtr3__u3u6ut9G5TVcytNFLLjg-XQab7pI0Sto1AC9eClksrwjR20giEEK4LRPABq36soo-Io5Tn7eNj7VPLvBWl2Y6KAu52fMC_kLCirjHodFGCt1bqroDmAoWSigoN7Kmn0Ze84uBfVbuv-qnYvqh3wGn0dvDxeWDYjxv9jR7cV-HAEPAW_G4qfQqJ_nABhpAVTuS8HDqu454TFUUg4BYypYJhdzOm1X_4A9h2gAQ</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Yang, S.Y.</creator><creator>Jian, Z.F.</creator><creator>Chieh, J.J.</creator><creator>Horng, H.E.</creator><creator>Yang, H.C.</creator><creator>Huang, I.J.</creator><creator>Hong, Chin-Yih</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20080501</creationdate><title>Wash-free, antibody-assisted magnetoreduction assays of orchid viruses</title><author>Yang, S.Y. ; Jian, Z.F. ; Chieh, J.J. ; Horng, H.E. ; Yang, H.C. ; Huang, I.J. ; Hong, Chin-Yih</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-36d2381f710851b9d0d55d242e5fa343461b7f540fcc72c651c0e5a94d3d41e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Antibodies, Viral - metabolism</topic><topic>Assay</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunoassay - methods</topic><topic>Magnetic beads</topic><topic>Magnetics</topic><topic>Magnetoreduction</topic><topic>Microbiology</topic><topic>Microspheres</topic><topic>Orchid virus</topic><topic>Orchidaceae</topic><topic>Orchidaceae - virology</topic><topic>Plant Viruses - isolation & purification</topic><topic>Techniques used in virology</topic><topic>Virology</topic><topic>Virology - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, S.Y.</creatorcontrib><creatorcontrib>Jian, Z.F.</creatorcontrib><creatorcontrib>Chieh, J.J.</creatorcontrib><creatorcontrib>Horng, H.E.</creatorcontrib><creatorcontrib>Yang, H.C.</creatorcontrib><creatorcontrib>Huang, I.J.</creatorcontrib><creatorcontrib>Hong, Chin-Yih</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, S.Y.</au><au>Jian, Z.F.</au><au>Chieh, J.J.</au><au>Horng, H.E.</au><au>Yang, H.C.</au><au>Huang, I.J.</au><au>Hong, Chin-Yih</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Wash-free, antibody-assisted magnetoreduction assays of orchid viruses</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>149</volume><issue>2</issue><spage>334</spage><epage>337</epage><pages>334-337</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>This study demonstrates the feasibility of wash-free magnetoreduction assays (MRA) of orchid viruses. A magnetic reagent, consisting of magnetic beads coated with antibodies and dispersed in water, was synthesized. By using a mixed-frequency alternative-current (ac) magnetosusceptometer, differences in the magnetic susceptibilities of the magnetic reagent before and after the addition of orchid solutions were measured. The results show significant advantages for MRA of orchid viruses.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>18367255</pmid><doi>10.1016/j.jviromet.2008.01.019</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-0934 |
| ispartof | Journal of virological methods, 2008-05, Vol.149 (2), p.334-337 |
| issn | 0166-0934 1879-0984 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70474643 |
| source | ScienceDirect Journals |
| subjects | Antibodies, Viral - metabolism Assay Biological and medical sciences Fundamental and applied biological sciences. Psychology Immunoassay - methods Magnetic beads Magnetics Magnetoreduction Microbiology Microspheres Orchid virus Orchidaceae Orchidaceae - virology Plant Viruses - isolation & purification Techniques used in virology Virology Virology - methods |
| title | Wash-free, antibody-assisted magnetoreduction assays of orchid viruses |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T06%3A00%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Wash-free,%20antibody-assisted%20magnetoreduction%20assays%20of%20orchid%20viruses&rft.jtitle=Journal%20of%20virological%20methods&rft.au=Yang,%20S.Y.&rft.date=2008-05-01&rft.volume=149&rft.issue=2&rft.spage=334&rft.epage=337&rft.pages=334-337&rft.issn=0166-0934&rft.eissn=1879-0984&rft.coden=JVMEDH&rft_id=info:doi/10.1016/j.jviromet.2008.01.019&rft_dat=%3Cproquest_cross%3E20777558%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c475t-36d2381f710851b9d0d55d242e5fa343461b7f540fcc72c651c0e5a94d3d41e33%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=20777558&rft_id=info:pmid/18367255&rfr_iscdi=true |