Unconventional secretion of fibroblast growth factor 2 and galectin-1 does not require shedding of plasma membrane-derived vesicles

Various molecular mechanisms of unconventional secretion of fibroblast growth factor 2 and galectin-1 have been proposed. A non-vesicular pathway that is based on direct translocation across the plasma membrane has been described. In other studies, however, release into the extracellular space of ce...

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Bibliographic Details
Published in:FEBS letters 2008-04, Vol.582 (9), p.1362-1368
Main Authors: Seelenmeyer, Claudia, Stegmayer, Carolin, Nickel, Walter
Format: Article
Language:English
Subjects:
Amides - pharmacology
Animals
Cell Membrane - metabolism
CHO Cells
Cricetinae
Cricetulus
Fibroblast growth factor 2
Fibroblast Growth Factor 2 - secretion
Galectin 1 - secretion
Galectin-1
Non-classical export
Pyridines - pharmacology
Unconventional protein secretion
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Summary:Various molecular mechanisms of unconventional secretion of fibroblast growth factor 2 and galectin-1 have been proposed. A non-vesicular pathway that is based on direct translocation across the plasma membrane has been described. In other studies, however, release into the extracellular space of cell-derived vesicles was implicated in both FGF-2 and Gal-1 secretion. Such vesicles were proposed to originate either from plasma membrane shedding or by the release of exosomes. Employing an inhibitor of plasma membrane blebbing and based on a quantitative biochemical analysis of cell culture supernatants for vesicles potentially carrying FGF-2 or Gal-1, we demonstrate that both FGF-2 and Gal-1 are not exported by shedding of plasma membrane-derived vesicles.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2008.03.024